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Mechanisms of electrical remodeling in lipotoxic guinea pig heart.
Biochem Biophys Res Commun
November 2019
Cardiac Electrophysiology and Metabolism Research Group, VA New York Harbor Healthcare System, Research and Development Office, (151), 800 Poly Place, Brooklyn, NY, 11209, USA; Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY, USA; Department of Physiology & Cellular Biophysics, Columbia University, New York, NY, USA. Electronic address:
Objectives: To develop an adult guinea pig model of lipotoxicity and explore the underlying mechanisms associated with changes in the expression of the delayed rectifier potassium current (I).
Background: Lipotoxicity may represent a common link among metabolic disorders and a higher vulnerability to arrhythmias.
Methods: Whole-cell patch clamp, and palmitic acid (PA, a potent inducer of lipotoxicity), were used to assess mechanisms of short-term (∼50 days) high-fat diet (HFD) feeding on atrial electrophysiology in guinea pig hearts and myocytes.
Molecular mechanism of TMEM16A regulation: role of CaMKII and PP1/PP2A.
Am J Physiol Cell Physiol
December 2019
Department of Pharmacology, Center of Biomedical Research Excellence for Molecular and Cellular Signal Transduction in the Cardiovascular System, University of Nevada, Reno School of Medicine, Reno, Nevada.
This study explored the mechanism by which Ca-activated Cl channels (CaCCs) encoded by the gene are regulated by calmodulin-dependent protein kinase II (CaMKII) and protein phosphatases 1 (PP1) and 2A (PP2A). Ca-activated Cl currents () were recorded from HEK-293 cells expressing mouse TMEM16A. were evoked using a pipette solution in which free Ca concentration was clamped to 500 nM, in the presence (5 mM) or absence of ATP.
View Article and Find Full Text PDFPharmacogenomics of cantharidin in tumor cells.
Biochem Pharmacol
February 2014
Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany. Electronic address:
Cantharis vesicatoria (blister beetle) is used in Chinese medicine and has been categorized as highly toxic in the Chinese pharmacopeia. In Europe, Cantharis patches have been used since ages to treat various skin-related diseases. We investigated the cytotoxicity of the Cantharis ingredient, cantharidin, in 41 tumor cell lines (Oncotest panel) and compared the results with those of 60 cell lines of the National Cancer Institute, USA.
View Article and Find Full Text PDFCantharidin patches and intravenous administration of vitamin C in the concomitant treatment of herpes zoster: a case report.
Zhong Xi Yi Jie He Xue Bao
April 2011
PAEN Clinic of Integrative Medicine, Roemerstr. 1, D-56130 Bad Ems, Germany.
Complex phosphatase regulation of Ca2+-activated Cl- currents in pulmonary arterial smooth muscle cells.
J Biol Chem
November 2009
Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557, USA.
The present study was undertaken to determine whether the two ubiquitously expressed Ca(2+)-independent phosphatases PP1 and PP2A regulate Ca(2+)-activated Cl(-) currents (I(Cl(Ca))) elicited by 500 nM [Ca(2+)](i) in rabbit pulmonary artery (PA) myocytes dialyzed with or without 3 mM ATP. Reverse transcription-PCR experiments revealed the expression of PP1alpha, PP1beta/delta, PP1gamma, PP2Aalpha, PP2Abeta, PP2Balpha (calcineurin (CaN) Aalpha), and PP2Bbeta (CaN Abeta) but not PP2Bgamma (CaN Agamma) in rabbit PA. Western blot and immunofluorescence experiments confirmed the presence of all three PP1 isoforms and PP2A.
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