A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin-protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 μg/kg for DON and 64 and 40 μg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102835 | PMC |
| http://dx.doi.org/10.1007/s00216-011-4973-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dataset of polarimetric images of mechanically generated water surface waves coupled with surface elevation records by wave gauges linear array.
Data Brief
February 2025
Faculty of Civil and Environmental Engineering, Technion, Haifa 320003, Israel.
Effective spatio-temporal measurements of water surface elevation (water waves) in laboratory experiments are essential for scientific and engineering research. Existing techniques are often cumbersome, computationally heavy and generally suffer from limited wavenumber/frequency response. To address these challenges a novel method was developed, using polarization filter equipped camera as the main sensor and Machine Learning (ML) algorithms for data processing [1,2].
View Article and Find Full Text PDFMultidimensional structural analyses revealed a correlation between thalamic atrophy and white matter degeneration in idiopathic dystonia.
Brain Commun
January 2025
Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department and Key Discipline of Neurology, Guangzhou 510080, China.
Although aberrant changes in grey and white matter are core features of idiopathic dystonia, few studies have explored the correlation between grey and white matter changes in this disease. This study aimed to investigate the coupling correlation between morphological and microstructural alterations in patients with idiopathic dystonia. Structural T1 imaging and diffusion tensor imaging were performed on a relatively large cohort of patients.
View Article and Find Full Text PDFEnhancing transcranial ultrasound stimulation planning with MRI-derived skull masks: a comparative analysis with CT-based processing.
J Neural Eng
January 2025
Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Transcranial ultrasound stimulation (TUS) presents challenges in ultrasound wave transmission through the skull, affecting study outcomes due to aberration and attenuation. While planning strategies incorporating 3D computed tomography (CT) scans help mitigate these issues, they expose participants to radiation, which can raise ethical concerns. A solution involves generating skull masks from participants' anatomical magnetic resonance imaging (MRI).
View Article and Find Full Text PDFEvaluation of the Femoral Condyle Radius of Curvature at the Chondral Surface Shows Significant Correlation With the Anterior-Posterior Length.
Cartilage
January 2025
Department of Radiology, University of California, San Diego, La Jolla, CA, USA.
Background: Accurate donor-recipient matching of the femoral condyle radius of curvature (ROC) in osteochondral allograft (OCA) transplantation may aid in minimizing articular surface incongruities. Matching linear femorotibial dimensions, such as the femoral condyle anterior-posterior length (APL), femoral condyle width (lateral-medial length, LML), femoral hemicondyle width (HCW), and tibial plateau width (TPW), can provide similar results if they correlate well with ROC. This study investigates the relationship between femorotibial dimensions and ROC at the cartilage surface using magnetic resonance imaging (MRI).
View Article and Find Full Text PDFCT-sensitized nanoprobe for effective early diagnosis and treatment of pulmonary fibrosis.
J Nanobiotechnology
January 2025
Nurturing Center of Jiangsu Province for State Laboratory of AI Imaging & Interventional Radiology; Basic Medicine Research and Innovation Center of Ministry of Education, Medical School of Southeast University, 87 Dingjiaqiao, Nanjing, 210009, China.
Early diagnosis is critical for providing a timely window for effective therapy in pulmonary fibrosis (PF); however, achieving this remains a significant challenge. The distinct honeycombing patterns observed in computed tomography (CT) for the primary diagnosis of PF are typically only visible in patients with moderate to severe disease, often leading to missed opportunities for early intervention. In this study, we developed a nanoprobe designed to accumulate at fibroblastic foci and loaded with the CT sensitizer iodide to enable effective early diagnosis of PF.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!