Since its invention in 1930, Zernike phase contrast has been a pillar in optical microscopy and more recently in x-ray microscopy, in particular for low-absorption-contrast biological specimens. We experimentally demonstrate that hard-x-ray Zernike microscopy now reaches a lateral resolution below 30 nm while strongly enhancing the contrast, thus opening many new research opportunities in biomedicine and materials science.
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http://dx.doi.org/10.1364/OL.36.001269 | DOI Listing |
Microsc Microanal
January 2025
EMAT, University of Antwerp, Groenenborgerlaan 171 2020, Antwerp, Belgium.
The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern transmission electron microscopy. This is especially true for life science imaging, where the sample, rather than the instrument, still determines the resolution limit. Here, we explore whether we have to accept this or can progress further in this area.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, 14476, Potsdam, Germany.
Neurodegeneration in Huntington's disease (HD) is accompanied by the aggregation of fragments of the mutant huntingtin protein, a biomarker of disease progression. A particular pathogenic role has been attributed to the aggregation-prone huntingtin exon 1 (HTTex1), generated by aberrant splicing or proteolysis, and containing the expanded polyglutamine (polyQ) segment. Unlike amyloid fibrils from Parkinson's and Alzheimer's diseases, the atomic-level structure of HTTex1 fibrils has remained unknown, limiting diagnostic and treatment efforts.
View Article and Find Full Text PDFJACS Au
December 2024
Department of Chemistry, University of Antwerp, Antwerp 2020, Belgium.
Proton-coupled electron transfer (PCET) is a fundamental redox process and has clear advantages in selectively activating challenging C-H bonds in many biological processes. Intrigued by this activation process, we aimed to develop a facile PCET process in cancer cells by modulating proton tunneling. This approach should lead to the design of an alternative photodynamic therapy (PDT) that depletes the mitochondrial electron transport chain (ETC), the key redox regulator in cancer cells under hypoxia.
View Article and Find Full Text PDFPolym Chem
December 2024
Polymer Science, Zernike Institute for Advanced Materials, University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands
Microscopes generally achieve better 2D imaging compared to 3D, and super-resolution microscopes often aggravate such axial-lateral resolution mismatch. A prime example is vortex phase-based stimulated emission depletion (STED) fluorescence microscopy, which only narrows the point-spread function laterally, thereby increasing the point-spread function (PSF) anisotropy. In this study, we developed a semi-analytical theory based on the Nijboer-Zernike expansion, developed a set of metrics and performed experiments to establish the merits of the alternative, bivortex phase-based, coherent-hybrid STED.
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