The yeast Saccharomyces cerevisiae has the capacity to take up and assemble dozens of different overlapping DNA molecules in one transformation event. These DNA molecules can be single-stranded oligonucleotides, to produce gene-sized fragments, or double-stranded DNA fragments, to produce molecules up to hundreds of kilobases in length, including complete bacterial genomes. This unit presents protocols for designing the DNA molecules to be assembled, transforming them into yeast, and confirming their assembly.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/0471142727.mb0322s94 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Circulating Tumor DNA Testing in Curatively Resected Colorectal Cancer and Salvage Resection.
JAMA Netw Open
December 2024
Department of Medical Oncology and Therapeutics Research, City of Hope Comprehensive Cancer Center, Duarte, California.
Importance: Serial circulating tumor DNA (ctDNA) has emerged as a routine surveillance strategy for patients with resected colorectal cancer, but how serial ctDNA monitoring is associated with potential curative outcomes has not been formally assessed.
Objective: To examine whether there is a benefit of adding serial ctDNA assays to standard-of-care imaging surveillance for potential curative outcomes in patients with resected colorectal cancer.
Design, Setting, And Participants: In this single-center (City of Hope Comprehensive Cancer Center, Duarte, California), retrospective, case cohort study, patients with stage II to IV colorectal cancer underwent curative resection and were monitored with serial ctDNA assay and National Cancer Center Network (NCCN)-guided imaging surveillance from September 20, 2019, to April 3, 2024.
A Method for the Separation of Phytophthora Oosporesfrom Soil for DNA-Based Detection.
Methods Mol Biol
January 2025
School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Here, we present a protocol for the isolation and detection of Phytophthora oospores directly from soil samples. Our method incorporates a novel technique for isolating Phytophthora oospores using filter pouches and an improved DNA extraction procedure specifically designed for oospores. While we have primarily developed this protocol for detecting P.
View Article and Find Full Text PDFThe Use of eDNA Metabarcoding to Detect and Identify Phytophthora in Water Samples.
Methods Mol Biol
January 2025
The James Hutton Institute, Dundee, UK.
We describe a protocol to amplify DNA barcodes of known and unknown taxa of Phytophthora and related plant pathogenic oomycetes from a range of environments. The methods focus on sampling pathogen propagules from water using in situ sampling and filtration equipment and buffers that enable efficient storage and DNA extraction for later downstream processing.
View Article and Find Full Text PDFQuantification of Phytophthora palmivora Infection in Barley and Related Monocot Roots.
Methods Mol Biol
January 2025
Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Biotic stresses such as fungal pathogens significantly affect global crop yields. Understanding of the plant-pathogen interactions during root infection, especially in monocot crops, remains limited compared to fungal colonizations of dicots. The infection process of several cereal crop root-damaging fungi and oomycetes is highly similar to root infections by the pathogen model Phytophthora palmivora.
View Article and Find Full Text PDFPromoter Analysis and Dissection Using Reporter Genes, Comparative Genomics, and Gel Shift Assays in Phytophthora.
Methods Mol Biol
January 2025
Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.
Transcriptional regulation allows cells to execute developmental programs, maintain homeostasis, and respond to intra- and extracellular signals. Central to these processes are promoters, which in eukaryotes are sequences upstream of genes that bind transcription factors (TFs) and which recruit RNA polymerase to initiate mRNA synthesis. Valuable tools for studying promoters include reporter genes, which can be used to indicate when and where genes are activated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!