Dissolved organic matter (DOM) obtained from landfill leachates was separated into hydrophobic base, hydrophilic matter (HIM), hydrophobic acid (HOA), and hydrophobic neutral fractions. The composition and transformation of the DOM and its fractions were investigated. The results show that the DOM isolated from young, intermediate, and old landfill leachates were mainly composed of tyrosine-, tryptophan-, and humic- and fulvic-like substances, respectively. The primary fractions of the DOM in leachates were HOA and HIM. The HOA and HIM fractions from young leachates predominantly contained tryptophan- and tyrosine-like materials, respectively. The HOA fractions in intermediate and old leachates were mainly composed of humic- and fulvic-like materials, whereas the HIM fractions were dominated by tryptophan-like materials and humic- and fulvic-like substances. The hydrophobic organic fractions and humic- and fulvic-like substances increased with time, whereas the HIM and the tyrosine-like materials decreased during the landfill process, rendering biological processing of leachates ineffective.
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