AI Article Synopsis
- The study aimed to analyze the expression of MUC2 and MUC5AC mRNA in four colon cancer cell lines (Caco-2, HT29, LoVo, and LS174T) using quantitative real-time PCR.
- MUC2 levels were highest in LS174T cells, followed by LoVo, HT-29, and Caco-2, while MUC5AC levels were highest in Caco-2 cells, followed by HT-29, LS174T, and LoVo.
- Caco-2 and LS174T cells were identified as suitable in vitro models for colon cancer mucin expression studies, with distinct morphological and functional characteristics between the two cell types.
Article Abstract
To compare the differences in MUC2 and MUC5AC mRNA among four colon cancer cell lines and to identify the best in vitro models for studying mucin expression, quantitative real-time polymerase chain reaction was used to measure the expression of MUC2 and MUC5AC mRNA in Caco-2, HT29, LoVo, and LS174T cell lines. The levels of MUC2 mRNA expression in the four colon cancer cell lines ranked in order of mRNA abundance were: LS174T>LoVo>HT-29>Caco-2. In contrast to MUC2, the abundances of MUC5AC mRNA were in the order: Caco-2>HT-29>LS174T>LoVo. Caco-2 (highest level of MUC5AC mRNA) and LS174T (highest level of MUC2 mRNA) were used to investigate the phenotypes. Morphologically, Caco-2 cells were larger with low electron density mucus-storing vacuoles, many cell surface microvilli, and no obvious intercellular spaces between cells, compared to LS174T cells. The proliferative and invasive capacities of LS174T cells were significantly higher than those of Caco-2 cells. Caco-2 and LS174T cells provide excellent in vitro models for studying mucin expression in colon cancer.
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| http://dx.doi.org/10.1016/j.tice.2011.03.002 | DOI Listing |
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