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http://dx.doi.org/10.4161/gmic.1.6.13726 | DOI Listing |
Int J Biol Macromol
December 2024
State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address:
Protein crystallization is essential for determining the three-dimensional structures of biomacromolecules and advancing biopharmaceutical development, yet it remains a major challenge in structural biology due to common issues like slow nucleation rates and inconsistent crystal quality. Herein, a dual-drive crystallization (DDC) strategy, relying on a composite film of sodium alginate (SA) and hyaluronic acid (HA), is reported to synergistically regulate both protein adsorption and solution supersaturation. Driven by the electrostatic interactions of SA and the water absorption properties of HA, the SA/HA film achieves enhanced crystallization efficiency and controlled crystal quality mainly.
View Article and Find Full Text PDFSci Rep
December 2024
Weifang University of Science and Technology, Jinguang Road No. 1299, Weifang City, Shandong Province, China.
Butyrate is one of the most abundant short-chain fatty acids (SCFAs), which are important metabolites of dietary fiber by fermentation of gut commensals, and has been shown to be vital in maintaining host health. The present study mainly investigated how sodium butyrate (NaB) supplementation in the diet with high proportion of soybean meal (SBM) affected turbot. Four experimental diets were formulated: (1) fish meal (FM) based diet (control group), (2) SBM protein replacing 45% FM protein in the diet (high SBM group), (3) 0.
View Article and Find Full Text PDFPol J Vet Sci
June 2024
College of Biological Engineering, Henan University of Technology, Zhengzhou, China.
Mannose oligosaccharide (MOS) has been shown to promote animal growth, maintain intestinal health, and activate the intestinal immune system. However, the question of whether MOS can stimulate the immune system and alleviate acetylsalicylic acid (ASA)-induced gut damage remains unresolved. The purpose of this study was to investigate the impact of MOS pretreatment on the immunological and anti-inflammatory capabilities of rats with ASA-induced intestinal injury.
View Article and Find Full Text PDFGut Microbes
December 2025
Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
The intracellular bacterium (Fn) mediates tumorigenesis and progression in colorectal cancer (CRC). However, the origin of intratumoral Fn and the role of Fn-infected immunocytes in the tumor microenvironment remain unclear. Here, we observed that Fn-infected neutrophils/macrophages (PMNs/MΦs), especially PMNs, accumulate in tumor tissues and fecal Fn abundance correlates positively with an abundance of blood PD-L1 PMNs in CRC patients.
View Article and Find Full Text PDFComp Biochem Physiol B Biochem Mol Biol
December 2024
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, Shandong Province 266109, China. Electronic address:
Lysozymes are crucial enzymes involved in the innate immune response against bacterial pathogens. In this study, we identified and characterized a goose-type lysozyme gene (SsLyG) from the black rockfish Sebastes schlegelii, an economically important aquaculture species. The deduced amino acid sequence of SsLyG contains 495 residues, which inculded a signal peptide, an immunoglobulin domain, and a goose egg-white lysozyme (GEWL) domain.
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