The natural ability of microorganisms for adhesion and biofilm formation on various surfaces is one of the factors causing the inefficiency of a disinfection agent, despite its proven activity in vitro. The aim of the study was to determine the effectiveness of disinfecting substances on bacterial biofilms formed on stainless steel surface. A universally applied disinfecting agent was used in the tests. Bacterial strains: Listeria innocua, Pseudomonas putida, Micrococcus luteus, Staphylococcus hominis strains, were isolated from food contact surfaces, after a cleaning and disinfection process. The disinfecting agent was a commercially available acid specimen based on hydrogen peroxide and peroxyacetic acid, the substance that was designed for food industry usage. Model tests were carried out on biofilm formed on stainless steel (type 304, no 4 finish). Biofilms were recorded by electron scanning microscope. The disinfecting agent in usable concentration, 0.5% and during 10 minutes was ineffective for biofilms. The reduction of cells in biofilms was only 1-2 logarithmic cycles. The use of the agent in higher concentration--1% for 30 minutes caused reduction of cell number by around 5 logarithmic cycles only in the case of one microorganism, M. luteus. For other types: L. innocua, P. putida, S. hominis, the requirements placed on disinfecting agents were not fulfilled. The results of experiments proved that bacterial biofilms are resistant to the disinfectant applied in its operational parameters. Disinfecting effectiveness was achieved after twofold increase of the agent's concentration.
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Cureus
December 2024
Anatomy/Embryology, Edward Via College of Osteopathic Medicine - Virginia, Blacksburg, USA.
Introduction Quaternary ammonium compounds (QACs) are the active ingredient in the majority of disinfectants approved for use against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19). Although widely used, they have been linked to infertility and birth defects in animals, and have been shown to increase proinflammatory cytokines, decrease mitochondrial function, and disrupt sterol biosynthetic pathways in a dose-dependent manner in humans. This study examined if there was an increased use of QAC-based disinfectants among healthcare settings in response to the COVID-19 pandemic and aims to bring to light the negative health outcomes that this rise in QAC exposure may pose.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata, 700106, India.
The conversion of solar energy into chemical energy or high-value chemicals has attracted considerable research interest in the context of the global energy crisis. Hydrogen peroxide (HO) is a versatile and powerful oxidizing agent widely used in chemical synthesis and medical disinfection. HO also serves as a clean energy source in fuel cells, generating electricity with zero-carbon emissions.
View Article and Find Full Text PDFJ Toxicol Sci
January 2025
Laboratory of Health Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University.
A representative surfactant, benzalkonium chloride (BAC) is used as a disinfectant, but sometimes causes serious side effects, including lung disorders such as interstitial pneumonia. However, its pathogenic mechanisms remain unexplained. In this study, we identified a novel mechanism by which BAC initiates inflammatory responses that may be responsible for its side effects.
View Article and Find Full Text PDFN Engl J Med
January 2025
From the University of Queensland, Brisbane, QLD, Australia (A.J.U., D.A., T.M.K., N.M., N.R., P.L.-A., V.G., A.C., P.M., C.M.R., P.N.A.H.); Children's Health Queensland Hospital and Health Service, Brisbane, QLD, Australia (A.J.U., T.M.K., P.L.-A., V.G.); Griffith University, Brisbane, QLD, Australia (A.J.U., D.A., T.M.K., R.M.W., N.M., A.C.B., R.R., J.B., V.C., C.M.R., R.S.W.); Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia (A.J.U., T.M.K., N.M., K.S., A.C., P.N.A.H.); Princess Alexandra Hospital, Brisbane, QLD, Australia (R.M.W., B.P., N.R., J.L., C.B., P.M.); University of Colorado, Denver (V.C.); and Metro North Health, Brisbane, QLD, Australia (C.M.R.).
Background: New catheter materials for peripherally inserted central catheters (PICCs) may reduce the risk of device failure due to infectious, thrombotic, and catheter occlusion events. However, data from randomized trials comparing these catheters are lacking.
Methods: We conducted a randomized, controlled, superiority trial in three Australian tertiary hospitals.
J Appl Toxicol
January 2025
Laboratorio de Genética y Toxicología Ambiental-Banco de Moscas, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, Mexico.
N-Nitrosodimethylamine (NDMA) is a known water disinfection byproduct (DBP) characterized as a potent hepatotoxin, promutagen, and probable human carcinogen; this is because of the metabolites associated with its biotransformation. The metabolism of NDMA produces formaldehyde, another alkylating agent and DBP. Both compounds are generated from natural and anthropogenic sources, but the safety restrictions applied to NDMA do not extend to the uses of formaldehyde.
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