Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528100 | PMC |
| http://dx.doi.org/10.1007/978-1-61779-092-8_5 | DOI Listing |
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