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Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I.
Neuron
December 2002
Department of Biochemistry, University of California, San Francisco, 94143, USA.
We demonstrate a noninvasive technique for protein photoinactivation using a transgenically encoded tag. A tetracysteine motif that binds the membrane-permeable fluorescein derivative 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein (FlAsH) was engineered into synaptotagmin I (Syt I4C). Neuronally expressed Syt I4C rescues the syt I null mutation, can be visualized after FlAsH labeling, and is normally distributed at the Drosophila neuromuscular synapse.
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