Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/HP.0b013e31820c6657 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of Enrichment Approaches for the Study of the Viromes in Mollusk Species.
Food Environ Virol
January 2025
Institute of Human Virology, Department of Pathogen Biology and Biosecurity, and Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
Invasive alien species such as freshwater snails have significantly affected the food, environment, and the health of humans and animals, which have unfortunately received insufficient attention. To facilitate the study of viromes in snail species, we compared the enrichment effect of cesium chloride (CsCl) and sucrose density gradient ultracentrifugations in the recovery of diverse viruses in Pomacea canaliculata and Achatina fulica. First, we showed that CsCl-based ultracentrifugation enriched more virus contigs and reduced the nucleic acid background of the Pomacea canaliculata and was thus beneficial for virus recovery.
View Article and Find Full Text PDFThe molecular structure of leaf starch from three cereal crops.
Carbohydr Polym
March 2025
Jiangsu Key Laboratory of Crop Genetics and Physiology/ State Key Laboratory of Hybrid Rice, College of Agriculture, Yangzhou University, Yangzhou 225009, China; Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/ Key Laboratory of Plant Functional Genomics, Ministry of Education/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China; Center for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address:
Plants produce storage and transient starches in seeds and in leaves, respectively. Understanding molecular fine structure and synthesis of transient starch can help improve plant quality (e.g.
View Article and Find Full Text PDFCounterintuitive DNA destabilization by monovalent salt at high concentrations due to overcharging.
Nat Commun
January 2025
Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, China.
Monovalent salts are generally believed to stabilize DNA duplex by weakening inter-strand electrostatic repulsion. Unexpectedly, our force-induced hairpin unzipping experiments and thermal melting experiments show that LiCl, NaCl, KCl, RbCl, and CsCl at concentrations beyond ~1 M destabilize DNA, RNA, and RNA-DNA duplexes. The two types of experiments yield different changes in free energy during melting, while the results that high concentration monovalent salts destabilize duplexes are common.
View Article and Find Full Text PDFTutorial on Describing, Classifying, and Visualizing Common Crystal Structures in Nanoscale Materials Systems.
ACS Nanosci Au
October 2024
Department of Chemistry, Department of Chemical Engineering, and Materials Research Institute, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.
Crystal structures underpin many aspects of nanoscience and technology, from the arrangements of atoms in nanoscale materials to the ways in which nanoscale materials form and grow to the structures formed when nanoscale materials interact with each other and assemble. The impacts of crystal structures and their relationships to one another in nanoscale materials systems are vast. This Tutorial provides nanoscience researchers with highlights of many crystal structures that are commonly observed in nanoscale materials systems, as well as an overview of the tools and concepts that help to derive, describe, visualize, and rationalize key structural features.
View Article and Find Full Text PDFIn-Depth Comparison of Adeno-Associated Virus Containing Fractions after CsCl Ultracentrifugation Gradient Separation.
Viruses
July 2024
National Institute of Biology, Večna pot 121, 1000 Ljubljana, Slovenia.
Recombinant adeno-associated viruses (rAAVs) play a pivotal role in the treatment of genetic diseases. However, current production and purification processes yield AAV-based preparations that often contain unwanted empty, partially filled or damaged viral particles and impurities, including residual host cell DNA and proteins, plasmid DNA, and viral aggregates. To precisely understand the composition of AAV preparations, we systematically compared four different single-stranded AAV (ssAAV) and self-complementary (scAAV) fractions extracted from the CsCl ultracentrifugation gradient using established methods (transduction efficiency, analytical ultracentrifugation (AUC), quantitative and digital droplet PCR (qPCR and ddPCR), transmission electron microscopy (TEM) and enzyme-linked immunosorbent assay (ELISA)) alongside newer techniques (multiplex ddPCR, multi-angle light-scattering coupled to size-exclusion chromatography (SEC-MALS), multi-angle dynamic light scattering (MADLS), and high-throughput sequencing (HTS)).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!