AI Article Synopsis
- Orbiviruses can infect various hosts, including humans, but detecting them is challenging due to their diversity.
- A new simple method using consensus reverse transcription PCR (RT-PCR) focuses on the polymerase gene for better recognition and characterization of these viruses.
- The process involves automated online sequence analysis to classify the resulting amplification products phylogenetically.
Article Abstract
Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3122741 | PMC |
| http://dx.doi.org/10.1128/JCM.00337-11 | DOI Listing |
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