We describe an optomagnetic bionanotechnology for rapid and sensitive solution-based affinity assays. Nanoactuators made from bioactive magnetic nanoparticles undergo rotational motion in the volume of a fluid under frequency-controlled magnetic actuation. The nanoactuators show a time-dependent scattering cross-section to an incoming light beam. We demonstrate that the temporal behavior of the scattered light intensity relates to the number, the magnetic properties and the size distribution of the nanoactuators, independently revealing the average value and variation in the magnetic properties of the nanoparticles as well as the concentration of nanoactuators. The method is applied to detect biomolecules in fluid by interparticle binding. In a total assay time of less than 3 min, we demonstrate a limit of detection lower than 400 fM in buffer and 5 pM in human plasma.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/nl200384p | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
TaqMan-based quantitative real-time polymerase chain reaction assay to detect porcine circovirus-like virus.
Pol J Vet Sci
December 2024
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, PR China. Email:
The aim of this study was to develop a rapid, sensitive and highly specific TaqMan quantitative real-time polymerase chain reaction PCR (qPCR) assay for porcine circovirus-like virus (PCLV). The primers and probe were designed based on the conserved regions of the PCLV ORF4 gene. The assay has a good detection performance (y=-3.
View Article and Find Full Text PDFElectrochemical Biosensors for Cancer Diagnosis: Multitarget Analysis to Present Molecular Characteristics of Tumor Heterogeneity.
JACS Au
December 2024
Center for Molecular Recognition and Biosensing, Shanghai Engineering Research Center of Organ Repair, Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), School of Life Sciences, Shanghai University, Shanghai 200444, China.
Electrochemical biosensors are gaining attention as powerful tools in cancer diagnosis, particularly in liquid biopsy, due to their high efficiency, rapid response, exceptional sensitivity, and specificity. However, the complexity of intra- and intertumor heterogeneity, with variations in genetic and protein expression profiles and epigenetic modifications, makes electrochemical biosensors susceptible to false-positive or false-negative diagnostic outcomes. To address this challenge, there is growing interest in simultaneously analyzing multiple biomarkers to reveal molecular characteristics of tumor heterogeneity for precise cancer diagnosis.
View Article and Find Full Text PDFRapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring.
Chem Biomed Imaging
December 2024
College of Chemistry and Materials Science, Jinan University, Guangzhou, Guangdong 510632, China.
The large-scale preparation of fluorescent nanomaterials with laboratory-relevant chemical and optical properties will greatly forward their consumer market applications; however, it still remains challenging. In this work, a universal strategy was developed for the rapid and large-scale synthesis of fluorescent sulfur quantum dots that recently has drawn great attention because of their unique optical characteristics. From the fact that empty 3d orbitals of sulfide species are able to bind with lone-pair π electrons of the heteroatomic groups, many amino-group containing compounds, such as amino acid and polyethylenimine molecules, were exploited to synthesize sulfur quantum dots.
View Article and Find Full Text PDFA high-throughput, turbulent-mixing, condensation aerosol concentrator for direct aerosol collection as a liquid suspension.
J Aerosol Sci
November 2024
National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Cincinnati, OH, 45226, United States.
Trace measurement of aerosol chemical composition in workplace atmospheres requires the development of high-throughput aerosol collectors that are compact, hand-portable, and can be operated using personal pumps. We describe the design and characterization of a compact, high flow, Turbulent-mixing Condensation Aerosol-in-Liquid Concentrator (TCALC) that allows direct collection of aerosols as liquid suspensions, for off-line chemical, biological, or microscopy analysis. The TCALC unit, measuring approximately 12 × 16 × 18 cm, operates at an aerosol sample flowrate of up to 10 L min, using rapid mixing of a hot flow saturated with water vapor and a cold aerosol sample flow, thereby promoting condensational growth of aerosol particles.
View Article and Find Full Text PDFAn Autocatalytic Platform Combining a Nonlinear Hybridization Chain Reaction and DNAzyme to Detect microRNA.
Bio Protoc
December 2024
Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China.
MicroRNAs (miRNAs) are small, non-coding RNAs that play pivotal roles in gene regulation; they are increasingly recognized as vital biomarkers for various diseases, notably cancer. Conventional methods for miRNA detection, such as quantitative PCR and microarray analysis, often entail intricate sample preparation and lack the requisite sensitivity to detect low-abundance miRNAs like miRNA-21. This protocol presents an innovative approach that combines branched hybridization chain reaction (bHCR) with DNAzyme technology for the precise detection of miRNA-21.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!