AI Article Synopsis
- A new hydrogen peroxide (H2O2) biosensor was created by attaching hemoglobin to a carbon-gold composite (C@Au) surface, which was made using a specialized seed-growth technique.
- The composite's gold shell provides a large surface area for hemoglobin, leading to effective biosensor performance, with a detection capability ranging from 5.0 to 135 microM and a detection limit of 1.67 microM.
- The biosensor demonstrates good reproducibility and long-term stability, making this composite a strong option for future biosensor developments.
Article Abstract
A promising hydrogen peroxide (H2O2) biosensor was fabricated by the immobilization of hemoglobin (Hb) on C@Au composite surface. The composite with carbon spheres and gold shell (C@Au) was synthesized via the seed-growth assembly technique. The assembly of the gold shell on carbon sphere surfaces was characterized by scanning electron miscroscopy (SEM). Owing to the unique structure and large surface area of the gold shell, the composite offered an effective interface for the immobilization of hemoglobin to fabricate a H2O2 biosensor. The obtained biosensor showed a wide linear range from 5.0 microM to 135 microM with a detection limit of 1.67 microM at 3sigma, and the apparent Michaelis-Menten constant (Km(app)) of the immobilized Hb was calculated to be 88.6 microM. Moreover, the biosensor also exhibited good reproducibility and long-term stability. Therefore, this kind of composite can provide an ideal matrix for protein immobilization and biosensor fabrication.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1166/jnn.2011.2673 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!