Carotenoid biosynthesis is regulated by blue light during growth of Neurospora crassa mycelia. We have cloned the al-1 gene of N. crassa encoding the carotenoid-biosynthetic enzyme phytoene dehydrogenase and present an analysis of its structure and regulation. The gene encodes a 595-residue polypeptide that shows homology to two procaryotic carotenoid dehydrogenases. RNA measurements showed that the level of al-1 mRNA increased over 70-fold in photoinduced mycelia. Transcription run-on studies indicated that the al-1 gene was regulated at the level of initiation of transcription in response to photoinduction. The photoinduced increase of al-1 mRNA levels was not observed in two Neurospora mutants defective in all physiological photoresponses. Analysis of cosmid containing al-1 and of a translocation strain with a breakpoint within al-1 indicated that al-1 transcription proceeds towards the centromere of linkage group I of N. crassa.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC361171 | PMC |
| http://dx.doi.org/10.1128/mcb.10.10.5064-5070.1990 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells.
STAR Protoc
January 2025
Institute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA. Electronic address:
Hematopoietic stem cells (HSCs) generate blood and immune cells. Here, we present a protocol to differentiate human pluripotent stem cells (hPSCs) into hematopoietic progenitors that express the signature HSC transcription factors HLF, HOXA5, HOXA7, HOXA9, and HOXA10. hPSCs are dissociated, seeded, and then sequentially differentiated into posterior primitive streak, lateral mesoderm, artery endothelium, hemogenic endothelium, and hematopoietic progenitors through the sequential addition of defined, serum-free media.
View Article and Find Full Text PDFTracing human trait evolution through integrative genomics and temporal annotations.
Cell Genom
January 2025
Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia. Electronic address:
Understanding the evolution of human traits is a fundamental yet challenging question. In a recent Cell Genomics article, Kun et al. integrate large-scale genomic and phenotypic data, including deep-learning-derived imaging phenotypes, with temporal annotations to estimate the timing of evolutionary changes that led to differences in traits between modern humans and primates or hominin ancestors.
View Article and Find Full Text PDFProtocol for alleviating alcohol-induced conditioned place preference in mice using alternating bilateral sensory stimulation.
STAR Protoc
January 2025
School of Medicine, Wuhan University of Science and Technology, Wuhan 430030, China. Electronic address:
Alternating bilateral sensory stimulation (ABS) is a clinical physical therapy technique effective in treating post-traumatic stress disorder (PTSD). However, its utilization in treating conditions beyond PTSD remains limited. Here, we present a protocol to reduce ethanol-induced conditioned place preference (CPP) using 4 Hz ABS.
View Article and Find Full Text PDFProtocol for the fabrication of self-standing (nano)cellulose-based 3D scaffolds for tissue engineering.
STAR Protoc
January 2025
Graz University of Technology, Institute for Chemistry and Technology of Biobased System (IBioSys), Stremayrgasse 9, 8010 Graz, Austria; Institute of Automation, Faculty of Electrical Engineering and Computer Science, University of Maribor, Maribor, Slovenia; Members of the European Polysaccharide Network of Excellence (EPNOE).
Three-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying.
View Article and Find Full Text PDFProtocol for identifying Dicer as dsRNA binding and cleaving reagent in response to transfected dsRNA.
STAR Protoc
January 2025
CAS Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Electronic address:
Mammalian Dicer has been proved to be functional on double-stranded RNAs (dsRNAs) and involved in antiviral immunity or immune regulation. Here, we present a protocol for identifying Dicer as a dsRNA binding and cleaving factor to transfected dsRNA in cell lines, based on small RNA sequencing (RNA-seq) and dsRNA-immunoprecipitation (dsRNA-IP). We detail both experimental processes and analysis on small RNA-seq data.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!