AI Article Synopsis
- The Global Polio Laboratory Network employs PCR techniques to identify poliovirus types and strains during surveillance efforts.
- To enhance epidemiological insights, they sequence a key gene (VP1) from poliovirus samples, but quality sequencing can be challenging with mixed viral populations.
- To address this issue, specialized serotype-specific primers have been created to effectively amplify and sequence the VP1 regions of distinct poliovirus types in mixed samples.
Article Abstract
The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the ~900-nucleotide region encoding the major capsid protein, VP1. It is difficult to obtain quality sequence information when clinical or environmental samples contain poliovirus mixtures. As an alternative to conventional methods for resolving poliovirus mixtures, sets of serotype-specific primers were developed for amplifying and sequencing the VP1 regions of individual components of mixed populations of vaccine-vaccine, vaccine-wild, and wild-wild polioviruses.
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| http://dx.doi.org/10.1016/j.jviromet.2011.03.020 | DOI Listing |
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