AI Article Synopsis
- The study investigates the role of the Id-1 gene in the growth and invasion of adenoid cystic carcinoma cells, specifically in two cell lines (ACC-M and ACC-2).
- Using assays like immunofluorescence, RT-PCR, and Western blot, researchers found that Id-1 is expressed in both cell lines, with higher expression in ACC-M.
- After knocking down Id-1 expression, they observed a significant reduction in cell growth and invasion, especially in the ACC-M line, suggesting that targeting Id-1 could be a promising treatment approach for adenoid cystic carcinoma.
Article Abstract
Objective: To investigate the role of inhibitor of DNA binding-1 (Id-1) gene in adenoid cystic carcinoma cell growth and invasion behavior.
Methods: With salivary adenoid cystic carcinoma cell lines ACC-M and ACC-2, dedected Id-1 gene expression was screened with immunofluorescence assay. After Id-1 mRNA knocking-down using small interfering RNA, RT-PCR and Western blot were used to detect the different expressions before and after interference, and the growth of cells before and after interference was deceted using the MTT assay, and the cell invasion ability was checked with the use of Transwell chamber assay.
Results: Id-1 were both expressed in the ACC-M and ACC-2, and the expression in ACC-M was higher than that in ACC-2. After Id-1 RNA interference, the growth and invasiveness of ACC-M and ACC-2 were inhibited with the restrained degree in ACC-M much stronger than that in the ACC-2.
Conclusion: In view of the important role of Id-1 in the behavior of growth and invasion in ACC cell, interfering the expression of Id-1 gene is expected to be a novel and effective means for the treatment of adenoid cystic carcinoma.
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