Diet fermentability influences lactational responses to feeding corn distillers grains (CDG) to dairy cows. However, some measures of diet fermentability are inherently related to the concentration and characteristics of corn-based ingredients in the ration. Corn-based feeds have poor protein quality, unable to meet the essential AA requirements of lactating cows. We conducted a meta-analysis of treatment means (n=44) from the scientific literature to evaluate responses in milk yield (MY) and milk true protein concentration and yield to dietary CDG. The test variable was the difference in response between the CDG diet mean and the control diet mean (0% CDG) within experiment. Fixed variables were CDG concentration of the diet [% of dietary dry matter (DM)] and crude protein (CP) concentration and fractions of CP based on origin (corn-based versus non-corn-based feeds) of control and CDG diets. Diets with CDG ranged from 4 to 42% CDG, DM basis. Non-corn-based dietary CP averaged 6.3±3.32% of total DM. Milk yield and milk true protein yield responses to added CDG were maximized when approximately 8.5% of the total dietary DM was non-corn-based CP. Milk yield response peaked for higher-producing cows (>30.0 kg MY/cow per day) at 4.3% dietary corn-based CP, but decreased linearly for lower-producing cows (<30.0 kg MY/cow per day) as corn-based dietary CP increased. Milk true protein yield response decreased as corn-based dietary CP concentration increased but milk true protein concentration response was not decreased when CDG diets had more than 6.5% dietary non-corn-based CP. Overall, 8.5% dietary non-corn-based CP was necessary in lactation diets to maximize lactational responses to dietary CDG. The necessity of dietary non-corn-based CP to maximize milk and milk protein yields limits the amount of dietary corn-based CP, including that from CDG, which can be included in rations without overfeeding N.

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