AI Article Synopsis
- Nucleic acid hybridization is a technique used to identify DNA and RNA types based on their similarities and measure their relative amounts.
- In this process, single-stranded DNA or RNA is attached to a filter and then exposed to a radioactive probe that matches the nucleic acids of interest.
- After incubation, any unbound probe is washed away, while the hybridized probes stick, although they can be removed in later washing steps depending on conditions.
Article Abstract
Nucleic acid hybridization is a very potent technique that can be used for the identification of DNA and RNA species with varying degree of homology and for the estimation of relative amounts of nucleic acid with known homolgy. In most cases, single-stranded (ss) (denatured) DNA or RNA is bound to a filter support (e.g., nitrocellulose) and incubated with a radioactively labeled ss DNA or RNA fragment ("probe") complementary to the nucleic acids of interest. In subsequent washing steps the nonhybridized probe is removed. Hybridized probe will be retained, but may be washed off in further washing steps, depending on the ion concentration and temperature of the wash.
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| http://dx.doi.org/10.1385/0-89603-127-6:385 | DOI Listing |
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