The genomic library of Staphylococcus aureus O15 has been constructed on the EMBL-3 vector. The synthetic oligonucleotide probes to N- and C-end regions of alpha-hemolysin permitted identification of the recombinant bacteriophage clone RS-1 containing a gene for this protein. The restriction map of the cloned fragment has been constructed for restriction endonucleases SalGI, EcoRV, PstI, PvuII. Expression of the alpha-hemolysin gene in phagolysate of the recombinant clone RS-1 (1000 units per ml) has been demonstrated.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
alpha-hemolysin gene
8
staphylococcus aureus
8
clone rs-1
8
[cloning alpha-hemolysin
4
gene staphylococcus
4
aureus strain
4
strain 015]
4
015] genomic
4
genomic library
4
library staphylococcus
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!