Aim: To transfect Hlx into mouse dendritic cell line DC2.4 and observe the effect of hlx on function of dendritic cells.
Methods: The eukaryotic expression vector PIRES2-EGFP/Hlx was transfected into DC2.4 by liposomes. The transfection efficiency was identified through FACS. RT-PCR and Real-time PCR were used to test the transcription level of Hlx in DC2.4. Forty-eight hours after transfection, DC2.4 cells were studied for cytokine production, cell phenotype, phagocytosis, unilateral mixed lymphocyte reaction.
Results: The pIRES2-EGFP/Hlx vector was transfected into DC2.4 with the transfection efficiency of up to 60%. Highly expressed Hlx in DC2.4 increased the expression of maturation makers including CD80 and CD86, and major histocompatibility complex-II. Functional assay showed that over-expression of Hlx in DC2.4 increased the interleukin-12 transcription and decreased DC endocytosis. The Hlx modified DC2.4 highly expressed IL-10 and TGF-β at the same time. Furthermore, it was shown that in a unilateral mixed lymphocyte reaction model, Hlx modified DC2.4 inhibited proliferation of lymphocytes.
Conclusion: Transient over-expression of Hlx in DC2.4 promotes DC2.4 maturation and up-regulates IL-12, IL-10 and TGF-β expression. However, the Hlx modified DC2.4 cells functionally appear as regulatory dendritic cells.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Soluble serum VCAM-1, whole blood mRNA expression and treatment response in natalizumab-treated multiple sclerosis.
Mult Scler Relat Disord
November 2016
Danish Multiple Sclerosis Center, Department of neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
Background: Natalizumab reduces disease activity in multiple sclerosis (MS). Natalizumab binds to the very late antigen-4 and inhibits vascular cell adhesion molecule-1 (VCAM-1)-mediated transmigration of immune cells across the blood-brain-barrier. This is associated with decreased serum concentrations of soluble (s)VCAM-1 and an altered composition of immune cell-subsets in the blood.
View Article and Find Full Text PDFTBX21 and HLX1 polymorphisms influence cytokine secretion at birth.
PLoS One
June 2012
Department of Pulmonary and Allergy, University Childreńs Hospital Munich, Munich, Germany.
Background: TBX21 (T cell specific T-box transcription factor) and HLX1 (H.20-like homeobox 1) are crucial transcription factors of T(H)1-cells, inducing their differentiation and suppressing T(H)2 commitment, particularly important for early life immune development. This study investigated the influence of TBX21 and HLX1 single nucleotide polymorphisms (SNPs), which have previously been shown to be associated with asthma, on T(H)1/T(H)2 lineage cytokines at birth.
View Article and Find Full Text PDFResin-composite cytotoxicity varies with shade and irradiance.
Dent Mater
March 2012
Department of Conservative Dentistry, Jena University Hospital, Friedrich-Schiller-Universität Jena, An der alten Post 4, Jena D-07743, Germany.
Objectives: The study was aimed at investigating the cytotoxicity of different composites as a function of composite shade and the light curing unit (LCU) employed.
Methods: Non-polymerized and polymerized samples of the composites Grandio(®) (VOCO, Cuxhaven), Solitaire(®) (Heraeus Kulzer, Hanau) and Filtek Z 250(®) (3M/Espe, Seefeld) in two markedly differing shades (A2, C2) were prepared. Polymerization was performed with two LCUs: Heliolux II (Ivoclar/Vivadent, Ellwangen) and Swiss Master Light (EMS, Nyon, Switzerland).
[Functional study of transcription factor Hlx modified dendritic cell line DC2.4].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
March 2011
Zhenjiang Entry-exit Inspection and Quarantine Bureau, Zhenjiang 212008, China.
Aim: To transfect Hlx into mouse dendritic cell line DC2.4 and observe the effect of hlx on function of dendritic cells.
Methods: The eukaryotic expression vector PIRES2-EGFP/Hlx was transfected into DC2.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!