Aim: To study the immunogenicity of the therapeutic multi-epitope gene vaccine of Hepatitis B virus.
Methods: Multi-epitope gene of Hepatitis B virus was cloned into prokaryon expression vector pBAD/gIIIA to express a non-fusional antigen B-BPT and BALB/c mice were immunized with the antigen. Cytotoxic T-lymphocyte(CTL) was determined with CytoTox96 kit which quantitatively measures the lactate dehydrogenase(LDH) that is released on cell lysis. CD4(+);, CD8(+); T lymphocytes subsets in immunized mice was detected by using flow cytometry (FCM). Lymphocyte proliferation response was completed by MTT colorimetry.
Results: Injection of B-BPT elicited high-level of CTL response and also stimulus spleen lymphocytes to proliferate effectively.
Conclusion: B-BPT can induce specific cellular immune responses and may be a good candidate for therapeutic vaccine.
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