Aim: Investigate the effects of Rb1 on phagocytosis and the production of the pro-inflammatory cytokines and NO of murine macrophage, to elucidate the machanism of the immuno-regulating effect of Rb1.
Methods: The single cell suspension of murine peritoneal macrophages was prepared under sterile condition. Then, the drug toxicology of different concent rations of Rb1 on macrophages was measured by MTT assay. The phagocytosis of macrophages to microbeads was observed through flow cytometry(FCM). The amount of NO produced in macrophages was detected by Griess kit. Used Cytometric Bead Arry(CBA) kit to detect the production of cytokines of Macrophages stimulated by LPS.
Results: In a dose-dependent manner, Rb1(5, 10, 20 μmol/L) can significantly inhibit the phagocytose of macrophage stimulated by LPS, but improve this ability of unactivated macrophage (P<0.05). Rb1 can also reduce the production of NO by macrophage(P<0.01). Rb1 can regulate the production of cytokines of macrophage stimulated by LPS.
Conclusion: Rb1 can inhibit the transduction of LPS signals and regulate the content of cytokines, thus to decrease the production of NO. However, to the unactivated macrophages, Rb1 up-regulates phagocytic functions and promotes the innate immunity to resist invasion of pathogens.
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