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http://dx.doi.org/10.1038/leu.2011.42 | DOI Listing |
Toxicol Pathol
January 2025
Johnson & Johnson Innovative Medicine, La Jolla, California, USA.
Small interfering RNAs (siRNAs) have been successfully used as therapeutics to silence disease-causing genes when conjugated to ligands or formulated in lipid nanoparticles to target relevant cell types for efficacy while sparing other cells for safety. To support the development of new methods for delivery of siRNA therapeutics, we developed and characterized a panel of antibodies generated against chemically modified nucleotides used in therapeutic siRNA molecules, identifying a monoclonal antibody that detects a broad range of siRNA representing distinct sequences and modification patterns. By integrating this anti-siRNA antibody with additional reagents, we created a multiplex siRNA immunoassay that simultaneously quantifies siRNA uptake, trafficking, and silencing activity.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Marine Stewardship Council, London, UK.
Management of natural resources and environmental systems has often involved top-down approaches in which government agencies set and enforce regulations on extractive activities. More recently, market-driven approaches were introduced to incentivise producers to voluntarily engage in practices that align with management objectives and support regulations. For the first time, we compare government and voluntary approaches within fisheries management systems and quantify their relative influences on the sustainability status of fish populations.
View Article and Find Full Text PDFDev Biol
January 2025
Willamette UniversityDepartment of Biology, 900 State St, Salem, OR 97301, United States of America.
Mammalian lactation is a dynamic process that develops throughout the lifespan of an organism. Here we present a framework for a third semester core course in biology that centers course content on lactation allowing examination of the developmental process as a dynamic whole-body experience involving changes occurring at the molecular, cellular, and organ levels of organization. Inequitable economic, socio- and geopolitical systems structure social determinants of health, affecting rates of breastfeeding in human populations.
View Article and Find Full Text PDFPurpose: Both clinical knowledge and patient care ownership (PCO) are crucial to the provision of quality patient care and should be acquired during training. However, the association between these two concepts is under-examined. Here, we conducted a nationwide cross-sectional study to investigate the association between clinical knowledge and PCO among resident physicians in Japan.
View Article and Find Full Text PDFBehav Processes
January 2025
University of Coimbra, Department of Life Sciences, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal; University of Coimbra, Research Centre for Anthropology and Health, Department of Life Sciences, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal.
Zoo animals are regularly exposed to a plethora of sensorial stimuli beyond their control, which can adversely impact their behaviour and welfare, including unfamiliar faces, excessive noise and intrusive visitor interaction. Zoos have implemented various measures, such as enrichments and regulation of visitor behaviour, to mitigate these effects. However, guided tours have not been used to simultaneously control visitor behaviour and maintain animal welfare.
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