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Logical Analysis of Multiple miRNAs with Isothermal Molecular Classifiers Based on LATE-RCA.

Nano Lett

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Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.

Logical analysis of multiple-miRNA expression information and immediate output of diagnostic results facilitates early cancer detection. In this work, we constructed an isothermal molecular classifier capable of performing computations on multiple miRNAs and directly providing diagnosis results. First, we developed linear-after-the-exponential rolling circle amplification (LATE-RCA), a nearly linear isothermal amplification that does not destroy the original quantitative information about miRNAs.

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Parents are essential members of the multidisciplinary teams supporting children with autism. Examining parents' priorities in the field of treatment options for children with autism can be very helpful to educators and therapists in the selection of appropriate treatments. The goal of the present study was to explore the treatment priorities which parents may have for their children with autism in Serbia.

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