Seven strains of bacteria which were separated from the active membrane on the mature manganese sand which are from different power plants. After identifying they are Leptothrix, Sphaerotilus and Siderocapsa. Studies show that the three strains of bacteria have a better removal of Fe2+, in which Siderocapsa No. 1 is the best. Leptothrix and Siderocapsa have a good removal of Mn2+ Siderocapsa No. 1 has the highest removal rate for Mn2+, Siderocapsa No. 2 is secondary. The mixture of separately cultured Leptothrix, Sphaerotilus, Siderocapsa No. 1 and Siderocapsa No. 2 which can remove iron and manganese were inoculated on the surface of manganese sand filter. Biofilm culturing with groundwater concentrations iron and manganese. The biological filter column has tended to maturate after 20d cultured. Test result indicates that the mature biological filter column can get rid of Fe2+ and Mn2+ nearly 100% and the biological filter column can run stably.
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