AI Article Synopsis
- SARS coronavirus is the only known highly pathogenic human coronavirus, causing a 2003 outbreak with a 10% fatality rate.
- Nsp16, a crucial protein for viral mRNA cap formation, functions as a methyltransferase but lacks known crystal structure.
- This paper details the expression, purification, and crystallization of nsp10 in complex with nsp16, achieving crystals that diffracted to 1.9 Å resolution, with structure determination ongoing.
Article Abstract
To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053173 | PMC |
| http://dx.doi.org/10.1107/S1744309111002867 | DOI Listing |
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