AI Article Synopsis
- The study identified seven new defective interfering (DI) RNA species of the porcine reproductive and respiratory syndrome virus (PRRSV) based on various analytical techniques.
- These DI RNAs exhibit significant deletions in a specific nucleotide range with characteristics that differ from previously reported DI RNAs, such as smaller deletion sizes and varying nucleotide repeats.
- The findings suggest that similarity-assisted RNA recombination is likely a key mechanism for the creation of DI RNAs in PRRSV and possibly other related viruses.
Article Abstract
In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses.
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| http://dx.doi.org/10.1016/j.virusres.2011.03.001 | DOI Listing |
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