The authors shows their experience in organizing a quality system in order to verify the competence of a laboratory and to ensure the validity of study results in the Sanitary Laboratory of the Branch of the Center of Hygiene and Epidemiology in the City of Moscow in the Central Administrative District of Moscow in accordance with the GOST requirements 17025-2006 "General Requirements for the Competence of Testing and Calibration Laboratories".
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Towards the standardization of Hydra vulgaris bioassay for toxicity assessments of liquid samples.
Ecotoxicol Environ Saf
December 2024
Aquatic Contaminants Research Division, Environment and Climate Change Canada, Montréal, Québec, Canada. Electronic address:
The Hydra vulgaris bioassay is recognized as sensitive invertebrate test species for toxicity assessment of real-life environmental mixtures for enforcement and monitoring investigations. The purpose of this study was to characterize the intra-laboratory variability, study the influence of environmental variables (temperature, luminosity, inter-individual and day of analysis) on ZnSO toxicity, a reference model toxicant for hydra. The sublethal (effect concentration for 50 % of hydra-EC50) and lethal (lethal concentration for 50 % of hydra-LC50) were determined based on characteristic morphological changes for this species.
View Article and Find Full Text PDFValidation, implementation and quality control of a Torque Teno Virus qPCR in a multinational clinical trial.
J Clin Virol
December 2024
Department of Microbiology and Infection Prevention, University of Groningen. University Medical Center Groningen, Groningen, The Netherlands.
Article Synopsis
- Immunosuppressive medication dosing after organ transplantation typically relies on therapeutic drug monitoring, but this method may not effectively predict rejection or infections.
- The TTVguideIT trial investigates using Torque Teno Virus (TTV) load measurement via qPCR as a potentially better alternative for dosing tacrolimus.
- Results demonstrated that the TTV R-GENE® assay showed excellent accuracy and consistency across participating labs, allowing for standardized TTV load measurement in clinical trials.
Inter-laboratory harmonization of microsphere immunoassays for SARS-CoV-2 antibody detection in dried blood spots and oral fluids.
bioRxiv
October 2024
Wadsworth Center, New York State Department of Health, Albany, NY.
Dried blood spots (DBS) and oral fluids (OF) are easily attainable biospecimen types that have enabled population scale antibody monitoring for SARS-CoV-2 exposure and vaccination. However, the degree to which the two different biospecimen types can be used interchangeably remains unclear. To begin to address this question, we generated contrived DBS (cDBS) and OF (cOF) from serum panels from SARS-CoV-2 infected, vaccinated, and uninfected individuals.
View Article and Find Full Text PDFModified radioimmunoassay versus ELISA to quantify anti-acetylcholine receptor antibodies in a mouse model of myasthenia gravis.
J Immunol Methods
November 2024
Neuromuscular Disorders Laboratory, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain; Center for Biomedical Network Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III, Madrid, Spain.
Article Synopsis
- A study was conducted to compare a modified radioimmunoassay (RIA) kit to two enzyme-linked immunosorbent assays (ELISA) methods for detecting anti-acetylcholine receptor (AChR) antibodies in a mouse model of myasthenia gravis (MG).
- The modified RIA showed high sensitivity (84.1%) and perfect specificity (100%), indicating its effectiveness in identifying AChR antibodies.
- The results suggest that using this modified RIA kit could streamline the antibody quantification process and minimize variability across different labs, as it doesn’t require time-consuming and costly antigen purification.
Development and validation of LC-MS/MS method for trace analysis of acrylamide, acrylic acid and N, N-methylene bis acrylamide in sandy loam soil.
J Chromatogr A
August 2024
Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India. Electronic address:
Article Synopsis
- Acrylamide and N,N-methylene bis-acrylamide are common compounds used to create super absorbent hydrogels, but concerns exist about their potential release of toxic substances into the soil as the hydrogels degrade.* -
- A method using Liquid Chromatography tandem mass spectrometry (LC-MS/MS) was developed for accurately measuring these compounds at trace levels in soil, particularly after the application of two types of hydrogels: Pusa Hydrogel and SPG 1118.* -
- The method proved effective with a limit of quantification for acrylamide, acrylic acid, and bis-acrylamide, showing good recovery rates and precision, while crops grown with the hydrogels showed no
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