The roundworm Caenorhabditis elegans is an effective model system for biological processes such as immunity, behavior, and metabolism. Robotic sample preparation together with automated microscopy and image analysis has recently enabled high-throughput screening experiments using C. elegans. So far, such experiments have been limited to per-image measurements due to the tendency of the worms to cluster, which prevents extracting features from individual animals.We present a novel approach for the extraction of individual C. elegans from clusters of worms in high-throughput microscopy images. The key ideas are the construction of a low-dimensional shape-descriptor space and the definition of a probability measure on it. Promising segmentation results are shown.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048333 | PMC |
| http://dx.doi.org/10.1109/ISBI.2010.5490286 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Functional classification of tauopathy strains reveals the role of protofilament core residues.
Sci Adv
January 2025
Center for Alzheimer's and Neurodegenerative Diseases, Peter O'Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Distinct tau amyloid assemblies underlie diverse tauopathies but defy rapid classification. Cell and animal experiments indicate tau functions as a prion, as different strains propagated in cells cause unique, transmissible neuropathology after inoculation. Strain amplification requires compatibility of the monomer and amyloid template.
View Article and Find Full Text PDFUnfolding of von Willebrand Factor Type D Like Domains Promotes Mucin Adhesion.
Nano Lett
January 2025
Department of Chemistry and Pharmacy, Institute of Physical Chemistry, University of Freiburg, Albertstr. 21, 79104 Freiburg, Germany.
Mucins are the macromolecular key components of mucus. On wet epithelia of mammals, mucin solutions and gels act as powerful biolubricants and reduce friction and wear by generating a sacrificial layer and establishing hydration lubrication. Yet the structure-function relationship of mucin adhesion and lubrication remains elusive.
View Article and Find Full Text PDFThe neonate respiratory microbiome.
Acta Physiol (Oxf)
February 2025
Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Hannover, Germany.
Over the past two decades, it has become clear that against earlier assumptions, the respiratory tract is regularly populated by a variety of microbiota even down to the lowest parts of the lungs. New methods and technologies revealed distinct microbiome compositions and developmental trajectories in the differing parts of the respiratory tract of neonates and infants. In this review, we describe the current understanding of respiratory microbiota development in human neonates and highlight multiple factors that have been identified to impact human respiratory microbiome development including gestational age, mode of delivery, diet, antibiotic treatment, and early infections.
View Article and Find Full Text PDFGeographic origin and evolution of dengue virus serotypes 1 and 3 circulating in Africa.
Virus Evol
December 2024
Department of Epidemiology and Population Health, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, United States.
Despite the increasing burden of dengue in Kenya and Africa, the introduction and expansion of the virus in the region remain poorly understood. The objective of this study is to examine the genetic diversity and evolutionary histories of dengue virus (DENV) serotypes 1 and 3 in Kenya and contextualize their circulation within circulation dynamics in the broader African region. Viral RNA was extracted from samples collected from a cohort of febrile patients recruited at clinical sites in Kenya from 2013 to 2022.
View Article and Find Full Text PDFAtomically resolved imaging of radiation-sensitive metal-organic frameworks via electron ptychography.
Nat Commun
January 2025
Center for Electron Microscopy, South China University of Technology, Guangzhou, China.
Electron ptychography, recognized as an ideal technique for low-dose imaging, consistently achieves deep sub-angstrom resolution at electron doses of several thousand electrons per square angstrom (e/Å) or higher. Despite its proven efficacy, the application of electron ptychography at even lower doses-necessary for materials highly sensitive to electron beams-raises questions regarding its feasibility and the attainable resolution under such stringent conditions. Herein, we demonstrate the implementation of near-atomic-resolution ( ~ 2 Å) electron ptychography reconstruction at electron doses as low as ~100 e/Å, for metal-organic frameworks (MOFs), which are known for their extreme sensitivity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!