AI Article Synopsis
- - Caveolin-1 (CAV1) levels are significantly high in Ewing's sarcoma (EWS), influencing the tumor's growth, drug resistance, and spread.
- - Prior studies showed that CAV1, secreted by certain cancer cells, is linked to tumor advancement, prompting an investigation into whether EWS cells also secrete CAV1.
- - Experiments confirmed that EWS cells not only secrete CAV1 but also absorb it from their environment, with extracellular CAV1 promoting increased cell proliferation, suggesting it may worsen the cancer's aggressive characteristics.
Article Abstract
Caveolin-1 (CAV1) is highly expressed in Ewing's sarcoma (EWS). We previously showed that increased cellular CAV1 is associated with the regulation of the tumorigenicity, drug resistance and metastatic ability of EWS cells. Because several studies reported that melanoma and prostate cancer cells, which express relatively high CAV1 levels, secrete CAV1, and that secreted CAV1 is associated with tumor progression, our study explored the possibility that EWS cells also secreted CAV1 and that secreted CAV1 may contribute to EWS pathobiology. Results from experiments involving the ectopic expression of a Myc-tagged CAV1 protein in EWS cells as well as the supplementation of culture media with purified CAV1 protein followed by its intracellular localization using immunofluorescence demonstrated that EWS cells secrete CAV1, that they are able to take up the secreted protein, and that extracellular CAV1 enhances EWS cell proliferation. These findings strongly support the notion that secreted CAV1 may also contribute to the malignant properties of EWS.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335731 | PMC |
| http://dx.doi.org/10.3892/ijo.2011.963 | DOI Listing |
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