AI Article Synopsis
- Protein phosphorylation is a crucial modification that influences protein activity and characteristics inside cells.
- Phosphosite-specific antibodies can be created as polyclonals or monoclonals from various animal species, each with its advantages and drawbacks.
- Validating these antibodies' specificity is essential for their application in proteomics and disease profiling, utilizing techniques like arrays, flow cytometry, and imaging.
Article Abstract
Protein phosphorylation is a universal key posttranslational modification that affects the activity and other properties of intracellular proteins. Phosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is key for their use in proteomics and profiling of disease.
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| http://dx.doi.org/10.1007/978-1-61779-024-9_1 | DOI Listing |
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