Chin Med J (Engl)
Department of Orthopaedics, Affiliated No. 6 People's Hospital of Shanghai Jiao Tong University, Shanghai 200233, China.
Published: January 2011
Background: Previous studies have demonstrated increased functions of osteoblasts on nanophase materials compared to conventional ceramics or composites. Nanophase materials are unique materials that simulate dimensions of constituent components of bone as they possess particle or grain sizes less than 100 nm. However, to date, interactions of osteoblasts on nanophase materials compared to conventional metals remain to be elucidated. The objective of the present in vitro study was to synthesize nanophase metals (Ti6Al4V), characterize, and evaluate osteoblast functions on Ti6Al4V. Such metals in conventional form are widely used in orthopedic applications.
Methods: In this work, nanophase Ti6Al4V surfaces were processed by the severe plastic deformation (SPD) principle and used to investigate osteoblast long-term functions. Primary cultured osteoblasts from neonatal rat calvaria were cultured on both nanophase and conventional Ti6Al4V substrates. Cell proliferation, total protein content, and alkaline phosphatase (ALP) activity were evaluated after 1, 3, 7, 10 and 14 days. Calcium deposition, gene expression of type I collagen (Col-I), osteocalcin (OC), osteopontin (OP) and the production of insulin-like growth factor-I (IGF-I) and transforming growth factor-beta 1 (TGF-β1) were also investigated after 14 days of culture.
Results: Functions of osteoblasts including proliferation, synthesis of protein, and ALP activity were improved on the nanophase compared to the conventional Ti6Al4V. The expression of Col-I, OC and OP mRNA was also increased on nanophase Ti6Al4V after 14 days of culture. Calcium deposition was the same; the average number of the calcified nodules on the two Ti6Al4V surfaces was similar after 14 days of culture; however, highly significant size calcified nodules on the nanophase Ti6Al4V was observed. Of the growth factors examined, only TGF-β1 showed a difference in production on the nanophase surface.
Conclusion: Nanophase Ti6Al4V surfaces improve proliferation, differentiation and mineralization of osteoblast cells and should be further considered for orthopedic implant applications.
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J Nanosci Nanotechnol
February 2016
In this study, we prepared magnesium (Mg) doped nano-phase hydroxyapatite (HAp) films on the TiO2 nano-network surface using electrochemical deposition method. Ti-6Al-4V ELI surface was anodized in 5 M NaOH solution at 0.3 A for 10 min.
View Article and Find Full Text PDFChin Med J (Engl)
January 2011
Department of Orthopaedics, Affiliated No. 6 People's Hospital of Shanghai Jiao Tong University, Shanghai 200233, China.
Background: Previous studies have demonstrated increased functions of osteoblasts on nanophase materials compared to conventional ceramics or composites. Nanophase materials are unique materials that simulate dimensions of constituent components of bone as they possess particle or grain sizes less than 100 nm. However, to date, interactions of osteoblasts on nanophase materials compared to conventional metals remain to be elucidated.
View Article and Find Full Text PDFZhonghua Yi Xue Za Zhi
July 2008
Sixth People's Hospital of Shanghai Jiaotong University, Shanghai 200233, China.
Objective: To evaluate the effect of nanophase Ti6Al4V substrates on the osseointegration in vivo.
Method: Novel nanophase Ti6Al4V substrates were prepared according to the severe plastic deformation principle. Eighteen New Zealand white rabbits were randomly divided into 3 equal groups with their trochanters of femur exposed and implanted with titanium substrate with common surface (Ti group), nanophase Ti6Al4V substrate (nano-Ti group), and hydroxyapatite-coated substrate (HA group) respectively.
Biomaterials
June 2006
Weldon School of Biomedical Engineering, Purdue University, 500 Central Drive, West Lafayette, IN 47907-2022, USA.
To date, long-term functions of osteoblasts leading to calcium and phosphorus mineral deposition on nanometals have not been determined. Nanometals are metals with constituent metal particles and/or surface features less than 100 nm in at least one dimension. For this reason, the objective of this in vitro study was to determine the amount of calcium and phosphorus mineral formation on microphase compared to nanophase Ti, Ti6Al4V, and CoCrMo cultured with and without osteoblasts (bone-forming cells).
View Article and Find Full Text PDFZhonghua Wai Ke Za Zhi
October 2005
Department of Orthopaedic, Ruijn Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Objective: To study the osseointegration of the nanophase hydroxyapatite biograde-coated implants and host bone.
Methods: Nanophase hydroxyapatite biograde-coated implants, hydroxyapatite biograde-coated implants and noncoated Ti-6Al-4V implants were inserted into both femoral of Beagle canines the tissue response, dynamic osteogensis and SEM were studied at 4, 8 and 12 weeks.
Results: The degradation of nanophase hydroxyapatite was slower than hydroxyapatite, dynamic osteogensis and the form of osteoblast were better than the control groups.
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