INTRODUCTIONThis RNA isolation procedure is suitable for tissue of almost any size, and is particularly useful for fetal organs or whole embryos from mice, as it can be performed in volumes as small as 0.5 mL. The minimum amount of tissue used should be ten 7.5-dpc (days post-coitum) embryos, one or two 8.5-dpc embryos, or approximately one-tenth of a 12.5-dpc embryo. If smaller amounts of tissue are homogenized in a 0.5-mL volume, the recovery of RNA may be less efficient.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1101/pdb.prot4773 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular Identification and Antifungal Susceptibility of Fusarium spp. Clinical Isolates.
Mycoses
January 2025
Clinical Microbiology Laboratory, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
Background: Accurate identification of Fusarium species requires molecular identification. Treating fusariosis is challenging due to widespread antifungal resistance, high rates of treatment failure, and insufficient information relating antifungal susceptibility to the clinical outcome. Despite recent outbreaks in Mexico, there is limited information on epidemiology and antifungal susceptibility testing (AST).
View Article and Find Full Text PDFAsymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students.
Folia Microbiol (Praha)
January 2025
Department of Public Health, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
Medical students are exposed to the hospital environment and patients during their studies, increasing the risk of exposure to virulent and antibiotic-resistant isolates of Staphylococcus aureus. The aim of the study is to determine the prevalence of Staphylococcus aureus among medical students who have varying levels of exposure to the hospital environment to provide valuable insights into the risk of colonization and transmission. Nasal swabs and fingerprints were obtained and cultured on a selective medium for staphylococci.
View Article and Find Full Text PDFOne is not like the other: health quality of life scores vary in neuromuscular EOS by diagnosis.
Spine Deform
January 2025
Department of Orthopaedic Surgery, Scottish Rite Hospital for Children, Dallas, TX, USA.
Purpose: The etiology of early-onset scoliosis (EOS) has been shown to significantly influence baseline parent-reported health-related quality of life (HrQOL). In combining these etiology groups, we obligatorily lump together many disparate diagnoses, particularly true in the neuromuscular (NM) cohort. We sought to evaluate the influence of underlying neuromuscular diagnosis on the HrQOL at 5 years following surgery for EOS.
View Article and Find Full Text PDFResurgence of common respiratory viruses and mycoplasma pneumoniae after ending the zero-COVID policy in Shanghai.
Sci Rep
January 2025
Department of Clinical Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, 399 Wanyuan Rd, Minhang District, Shanghai, 201102, China.
China has adhered to policies of zero-COVID for almost three years since the outbreak of COVID-19, which has remarkably affected the circulation of respiratory pathogens. However, China has begun to end the zero-COVID policies in late 2022. Here, we reported a resurgence of common respiratory viruses and Mycoplasma pneumoniae with unique epidemiological characteristics among children after ending the zero-COVID policy in Shanghai, China, 2023.
View Article and Find Full Text PDFAssessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study.
BMC Oral Health
January 2025
Clinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, 162, Poonamallee High Road, Velappanchavadi, Chennai, Tamil Nadu, 600077, India.
Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers in the world. The major etiological factors are considered to be tobacco and alcohol. However, the etiological factors for non-habit associated oral squamous cell carcinoma (NHOSCC) remains an enigma.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!