INTRODUCTIONThis protocol describes the electroporation of DNA constructs to drive in vivo gene expression in neurons during early chick development. Electroporation is a method of physically introducing DNA constructs into cells through the application of an electric field. This simple method is important as it allows the ectopic expression of transgenes with relative ease in most neurons.
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| http://dx.doi.org/10.1101/pdb.prot4788 | DOI Listing |
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