INTRODUCTIONRNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a method for collection of Drosophila embryos for RNA interference (RNAi) experiments. The embryos are collected in a simple, homemade apparatus, arrayed on prepared glass slides, and readied for injection. It is important to keep the embryos moist and oxygenated. Work expeditiously, because embryos should be injected within 30-60 min after collection.
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Article Synopsis
- Innovative strategies are needed to combat fungal pathogens for sustainable crop protection, with traditional fungicides facing resistance issues due to their single-target action.
- The study investigated the synergistic effects of chitosan (CS) and the fungicide azoxystrobin, finding a high synergy score that significantly improves antifungal efficacy.
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