AI Article Synopsis
- This paper outlines a microencapsulation process for spore crystal aggregates from Bacillus thuringiensis var. kurstaki HD-1 using emulsification/internal gelation.
- The resulting microcapsules, with diameters under 10 μm, effectively shielded the spore aggregates from harmful ultraviolet radiation, maintaining about 60% spore viability and 40% protein activity after exposure.
- The study suggests that these microcapsules could enhance the effectiveness and yield of Bt-HD1 in biological control applications when released into the environment.
Article Abstract
This paper describes a microencapsulation process of a spore crystal aggregate produced by Bacillus thuringiensis var. kurstaki HD-1. The methodology is based on the emulsification/internal gelation method, and was implemented to produce microcapsules of small diameter (< 10 μm) with the capacity to protect the spore crystal aggregate from extreme ultraviolet radiation. The diameter of microcapsules was in the range of 3.1 ± 0.2-6.8 ± 0.4 μm, which is considered adequate for biological control purposes. The protective effect of the alginate coat was verified by the remaining 60 ± 2% and 40 ± 1% of spore viability and protein activity, respectively, after UV-B radiation of 236 J, and with bioassays with Spodoptera frugiperda. It is expected that the protective effect of the alginate coat will improve the effectiveness of the Bt-HD1 formulated as small diameter microcapsules, and their yield, once they are released into the environment, will also be improved.
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| http://dx.doi.org/10.1007/s00449-011-0519-x | DOI Listing |
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