Preparations of calcium-tolerant cardiac myocytes for studies on intracellular calcium ([Ca(2+)](i)) signaling and contraction have been difficult owing to the susceptibility of these cells to the enzymatic digestion process. This often leads to the cells acquiring a bricklike contracted shape with a granular cytoplasm and membrane blebbing. A successful preparation results in single myocytes that do not show spontaneous contractions and that remain relaxed in Ca(2+)-containing buffers, indicating that the sarcolemmal membrane was not damaged during enzymatic digestion and led to membrane depolarization.
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