AI Article Synopsis
- Visceral leishmaniasis is prevalent in Brazil, especially in northern Minas Gerais, and this study aimed to identify natural infections of Lutzomyia longipalpis (sandflies) and the specific Leishmania species present in Janaúba.
- Researchers collected 1,550 female sandflies using light traps, grouping them for DNA extraction and amplification, and found an infection rate of 3.9% in the samples tested.
- The positive samples were similar to the Leishmania chagasi strain, indicating that L. longipalpis plays a significant role in the transmission of this disease in areas with high incidence of visceral leishmaniasis.
Article Abstract
Introduction: Visceral leishmaniasis has been notified in nearly all states of Brazil, and particularly in the north of Minas Gerais, where the disease is endemic. The aim of this study was to detect natural infection of Lutzomyia longipalpis and, through the PCR/RFLP technique, identify Leishmania species found in sandflies in the municipality of Janaúba.
Methods: Using light traps, 1,550 females of L. longipalpis were caught and grouped into pools of 10 specimens to be subjected to DNA extraction and amplification, by means of generic PCR and cacophony.
Results: Out of the 155 pools, six were positive for Leishmania sp., and thus the infection rate in the municipality was 3.9%. Through PCR/RFLP, the digestion pattern among the positive samples was found to be similar to that of the reference strain of Leishmania chagasi (MHOM/BR/74/PP75).
Conclusions: The detection of natural infection associated with studies on the epidemiology of visceral leishmaniasis suggests that L. longipalpis is involved in transmission of L. infantum chagasi in Janaúba, particularly in areas of intense transmission of visceral leishmaniasis.
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| http://dx.doi.org/10.1590/s0037-86822011000100014 | DOI Listing |
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