AI Article Synopsis
- Birds, particularly nestlings, are hard to identify by gender early on, making it challenging to manage unwanted chicks in hatcheries without welfare concerns regarding culling.
- A new method using Thymine-DNA Glycosylase (TDG) allows for sex determination in chickens without requiring PCR, effectively recognizing and correcting thymine/guanine mismatches in their DNA.
- Initial laboratory findings suggest that this technique can directly detect chicken gender through specific DNA sequences, and with further development, it may be usable outside of lab environments where PCR is impractical.
Article Abstract
1. Birds, especially nestlings, are generally difficult to sex by morphology and early detection of chick gender in ovo in the hatchery would facilitate removal of unwanted chicks and diminish welfare objections regarding culling after hatch. 2. We describe a method to determine chicken gender without the need for PCR via use of Thymine-DNA Glycosylase (TDG). TDG restores thymine (T)/guanine (G) mismatches to cytosine (C)/G. We show here, that like DNA Polymerase, TDG can recognise, bind and function on a primer hybridised to chicken genomic DNA. 3. The primer contained a T to mismatch a G in a chicken genomic template and the T/G was cleaved with high fidelity by TDG. Thus, the chicken genomic DNA can be identified without PCR amplification via direct and linear detection. Sensitivity was increased using gender specific sequences from the chicken genome. 4. Currently, these are laboratory results, but we anticipate that further development will allow this method to be used in non-laboratory settings, where PCR cannot be employed.
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| http://dx.doi.org/10.1080/00071668.2010.549665 | DOI Listing |
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