For many infectious agents, the detection of antibodies is critical for diagnosing, monitoring and understanding vaccine responses. To facilitate the highly quantitative and simultaneous analysis of antibodies against multiple proteins from infectious agents, we have developed Luciferase Immunoprecipitation Systems (LIPS) arrays. By configuring microtiter plates with multiple antigens and testing control and infected serum samples at one time in solution, LIPS arrays provided highly reproducible antibody titers to panels of antigens with a wide dynamic range of detection. While all serum samples showed similar positive and negative immunoreactivity with internal control antigens derived from Influenza and Renilla luciferase-alone protein, respectively, antibody titers to many HCV and HIV antigens were generally 10 to over 400-fold higher in the infected versus uninfected samples. Additional screening of 18 proteins from the EBV proteome with serum samples from healthy EBV-infected individuals showed statistically significant antibody titers to 50% of the proteins tested. Antibody titers for the different EBV antigens in the healthy EBV-infected individuals were markedly heterogeneous highlighting the complexity of host humoral responses. These results suggest that LIPS arrays offer a highly discriminating platform for simultaneously profiling a wide spectrum of antibodies associated with many infectious agents.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430135 | PMC |
| http://dx.doi.org/10.1039/c0mb00342e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Risk of adverse cardiovascular events based on common genetic variants in 8-year follow-up of the LIPIDOGEN2015 population using the polygenic risk score (PRS): study design and methodology.
Arch Med Sci
August 2024
Department of Family Medicine and Public Health, Faculty of Medicine, University of Opole, Poland.
Introduction: Classical risk factors such as hypertension, hypercholesterolemia, pre-diabetes, diabetes and obesity can predict adverse cardiovascular events, but they are less prognostic in patients aged < 60 years. Polygenic risk scores (PRS) can be effective in predicting adverse coronary events in younger and middle-aged patients. Our main aim is to assess the utility of a new PRS created for the Polish population in predicting mortality during an 8-year follow-up in the nationwide LIPIDOGEN2015 population.
View Article and Find Full Text PDFPrenatal diagnosis, ultrasound findings and pregnancy outcome of 7q11.23 deletion and duplication syndromes: what are the fetal features?
BMC Pregnancy Childbirth
November 2024
The Genetics Laboratory, Longgang District maternity & Child Healthcare Hospital of Shenzhen City (Longgang Maternity and Child Institute of Shantou University Medical College), Shenzhen, Guangdong, China.
Article Synopsis
- The study aimed to analyze ultrasound findings, SNP-array results, and pregnancy outcomes in fetuses with 7q11.23 deletions and duplications during the second and third trimesters, gathering comprehensive data from a Chinese medical center over several years.
- Seven fetuses with 7q11.23 deletions and six with duplications were diagnosed, revealing significant clinical abnormalities such as intrauterine growth restriction and various cardiovascular issues; the deletions were associated with more serious ultrasound findings.
- Most pregnancies (seven deletions and three duplications) were terminated due to the severe abnormalities discovered, indicating a high risk linked to these genetic conditions.
Prenatal diagnosis, ultrasound findings, and pregnancy outcome of 17q12 deletion and duplication syndromes: a retrospective case series.
Arch Gynecol Obstet
December 2024
Department of Community Center, Longgang District People's Hospital of Shenzhen City (The Second Affiliated Hospital of The Chinese University of Hong Kong, Shenzhen), Shenzhen, Guangdong, China.
Article Synopsis
- - The study examined ultrasound findings and SNP-array results for fetuses with 17q12 deletions and duplications during the second and third trimesters, focusing on prenatal characteristics and outcomes.
- - Data from 16 fetuses with deletions and 7 with duplications were analyzed, revealing significant abnormalities; deletions often led to renal issues, while duplications were associated with cardiovascular and gastrointestinal problems.
- - Out of the pregnancies, 9 were terminated, while 14 continued; some cases presented multiple severe structural malformations, highlighting the complexity of conditions associated with these genetic variations.
Compact Electron Paramagnetic Resonance on a Chip Spectrometer Using a Single Sided Permanent Magnet.
ACS Sens
October 2024
Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, 14109 Berlin, Germany.
Electron paramagnetic resonance (EPR) spectroscopy provides information about the physical and chemical properties of materials by detecting paramagnetic states. Conventional EPR measurements are performed in high resonator using large electromagnets which limits the available space for operando experiments. Here we present a solution toward a portable EPR sensor based on the combination of the EPR-on-a-Chip (EPRoC) and a single-sided permanent magnet.
View Article and Find Full Text PDFMulti-regional genomic and transcriptomic characterization of a melanoma-associated oral cavity cancer provide evidence for CASP8 alteration-mediated field cancerization.
Hum Genomics
September 2024
Biotechnology Research and Innovation Council, National Institute of Biomedical Genomics (BRIC-NIBMG), Kalyani, 741251, India.
Background: Precancerous and malignant tumours arise within the oral cavity from a predisposed "field" of epithelial cells upon exposure to carcinogenic stimulus. This phenomenon is known as "Field Cancerization". The molecular genomic and transcriptomic alterations that lead to field cancerization and tumour progression is unknown in Indian Oral squamous cell carcinoma (OSCC) patients.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!