Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations of the technique, irrelevant results might be trusted. To address this issue, we here propose a step-by-step quality control of the iTRAQ workflow. From sample preparation to final ratio calculation we provide metrics and techniques assessing the actual effectiveness of iTRAQ quantification as well as a novel method for more reliable protein ratio estimation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/pmic.201000711 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Efficient production system for hydrogel-based transparent soil for plant root observation.
Biotechniques
January 2025
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Observation of plant root morphology in soil is of fundamental importance in plant research, but the lack of transparency of the soil hampers direct observation of roots. One of the approaches to overcome this technical limitation is the use of "transparent soil" (TS), hydrogel-based beads produced by spherification of gelling agents. However, the production of TS by natural dripping of gelling solution can be labor intensive, time consuming and difficult to maintain consistent product quality.
View Article and Find Full Text PDFAssociations Between Muscle Thickness, Motor Function, and Echo Intensity in Community-Dwelling Older Japanese Men and Women.
Cureus
December 2024
Research Team for Human Care, Tokyo Metropolitan Institute for Geriatrics and Gerontology, Tokyo, JPN.
Purpose Muscle atrophy progresses with age. The motor function may be estimated by measuring the muscle mass; however, if muscle quality deteriorates due to an increase in connective tissue within the muscle, a decline in motor function may be missed by measuring muscle mass alone. Therefore, it is important to understand the relationship between muscle mass, muscle quality, and motor function.
View Article and Find Full Text PDFThe Efficacy of Repetitive Transorbital Alternating Current Stimulation (rtACS) in Patients With Optic Nerve Damage: A Systematic Review and Meta-Analysis.
Cureus
December 2024
Department of Ophthalmology, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Jeddah, SAU.
Optic nerve disorders significantly contribute to visual impairment with irreversible visual deficits. Current treatments have limited efficacy in resolving chronic visual deficits, necessitating novel therapeutic strategies. Neurorehabilitation techniques, including repetitive transorbital alternating current stimulation (rtACS), have emerged as promising approaches to restore lost visual function through the ability to modulate brain activity.
View Article and Find Full Text PDFEffects of coupled application of magnetoelectric activated water and amendments on photosynthetic physiological characteristics and yield of maize in arid regions.
Front Plant Sci
January 2025
State Key Laboratory of Eco-hydraulics in Northwest Arid Region, Xi'an University of Technology, Xi'an, China.
Seeking effective improvement agent control measures to enhance the photosynthetic physiological traits and yield levels of spring maize is crucial for efficient green agriculture in arid regions. Therefore, this study was conducted to clarify the effects of coupling improvement agents under magnetoelectric activated water irrigation conditions on the photosynthetic physiological traits, grain nutrients, and yield of spring maize in the arid region of northwest China. Field experiments were set up with three concentrations of growth regulators: 400 times (G1), 500 times (G2), and 600 times (G3), and three amounts of : 15 kg/ha (R1), 45 kg/ha (R2), and 75 kg/ha (R3), along with a control group CK, making a total of 10 treatments applied in the field experiment.
View Article and Find Full Text PDFUnlocking High-Throughput Plasma-Catalytic Low-Temperature Oxidation of -Hexane over Single-Atom Ag/MnO Catalysts.
JACS Au
January 2025
College of Chemical and Biological Engineering, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
The total oxidation of -hexane, a hazardous volatile organic compound (VOC) emitted by the pharmaceutical industry, presents a significant environmental challenge due to limited catalyst activity at low temperatures and poor stability at high temperatures. Here, we present a novel approach that overcomes these limitations by employing single-atom Ag/MnO catalysts coupled with nonthermal plasma (NTP). This strategy achieves exceptional performance in -hexane oxidation at low temperatures, demonstrating 96.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!