AI Article Synopsis
- The video explores using patient tissue as an ex vivo model for gene delivery studies, where fresh tissue from surgeries is cultured and sliced for experimentation.
- Gene delivery is assessed using bioluminescence imaging with the IVIS detection system, allowing for quick and precise measurement of gene expression in tissue slices without sacrificing them.
- This system can be applied to various tissue types, including both normal and cancerous tissue, offering a potential alternative to animal models and enhancing preclinical research before clinical trials.
Article Abstract
This video describes the use of patient tissue as an ex vivo model for the study of gene delivery. Fresh patient tissue obtained at the time of surgery is sliced and maintained in culture. The ex vivo model system allows for the physical delivery of genes into intact patient tissue and gene expression is analysed by bioluminescence imaging using the IVIS detection system. The bioluminescent detection system demonstrates rapid and accurate quantification of gene expression within individual slices without the need for tissue sacrifice. This slice tissue culture system may be used in a variety of tissue types including normal and malignant tissue and allows us to study the effects of the heterogeneous nature of intact tissue and the high degree of variability between individual patients. This model system could be used in certain situations as an alternative to animal models and as a complementary preclinical mode prior to entering clinical trial.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159673 | PMC |
| http://dx.doi.org/10.3791/2378 | DOI Listing |
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