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Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center. | LitMetric

AI Article Synopsis

  • This study explored whether freeze-dried bull spermatozoa could maintain their microtubule-organizing center (MTOC) function post-rehydration and intracytoplasmic sperm injection (ICSI).
  • Preliminary findings showed that the cleavage and blastocyst formation rates in FD-ICSI zygotes were significantly lower compared to control ICSI and IVF zygotes.
  • The main experiment indicated that sperm aster formation and microtubule assembly in FD-ICSI oocytes were similar to control ICSI, suggesting that freeze-drying does not negatively impact MTOC function in bull sperm.

Article Abstract

This study was designed to investigate whether freeze-dried (FD) bull spermatozoa maintained the function of the microtubule-organizing center (MTOC) after rehydration and intracytoplasmic sperm injection (ICSI). In a preliminary attempt, the cleavage and blastocyst formation rates in FD-ICSI zygotes (36 and 1%, respectively) were found to be considerably lower than those in control ICSI zygotes (67 and 21%, respectively) or in IVF zygotes (78 and 43%, respectively). An alkaline comet assay indicated that the DNA fragmentation index (length of comet tail % DNA liberated) was not significantly different between fresh and FD spermatozoa. In the main experiment, formation of sperm-asters in the FD-ICSI oocytes 7 h postinsemination occurred at a similar rate when compared with the control ICSI oocytes (41 vs. 49%). Among the oocytes exhibiting sperm aster formation, the extent of microtubule network assembly was comparable between the FD-ICSI and control ICSI groups. However, the MTOC of the ICSI oocytes was not as functional as that of IVF oocytes in terms of the aster formation rate (97%) and the fluorescent intensity of the microtubule network (2.0 folds). These results suggest that the freeze-drying process per se had no adverse effect on maintaining the MTOC function in bull spermatozoa.

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Source
http://dx.doi.org/10.1262/jrd.10-167nDOI Listing

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