The effect of corn stover pretreatment on glucose quantitation in hydrolysate using Raman spectroscopy is evaluated. Dilute sulfuric-acid pretreatment results in a 20 mg mL(-1) glucose limit of detection in hydrolysate. Soaking in aqueous ammonia pretreatment produces a 4 mg mL(-1) limit of detection. Water, ethanol or hexane extraction of corn stover reduces the spectral background that limits glucose detection in dilute acid hydrolysate. Additionally, a Raman spectroscopy multi-peak fitting method is presented to simultaneously measure glucose and xylose concentration in hydrolysate. This method yields a 6.1% average relative standard error at total saccharide concentrations above 45 mg mL(-1). When only cellulase is present, glucose and xylose yield were measured by Raman spectroscopy to be 32 ± 4 and 7.0 ± 0.8 mg mL(-1), respectively. When both cellulase and hemicellulase were present, xylose yield increased to 18.0 ± 0.5 mg mL(-1). Enzymatic or colorimetric assays confirmed the validity of the Raman spectroscopy results.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biortech.2011.01.043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Magnesium phosphates experienced high-temperature transition found on the CI-like carbonaceous chondrite Yamato 980115 by Raman microspectroscopy.
Anal Sci
January 2025
National Institute of Polar Research (NIPR), 10-3 Midori-Cho, Tachikawa, Tokyo, 190-8518, Japan.
Magnesium phosphate grains, minor accessory minerals found on the primitive meteorite Yamato 980115 (Y 980115), were investigated by Raman microspectroscopy. All magnesium phosphate grains found in the present study can be assigned to farringtonite, dehydrated magnesium phosphate Mg(PO)-I. Since the Mg(PO)-I is generally formed via the irreversible thermal transition from the polymorphs of Mg(PO)-II and -III at above 750-800 degree Celsius, we can infer that the parent body of the Y 980115 meteorites experienced thermal alteration with such a high temperature.
View Article and Find Full Text PDFIn Operando Raman Spectroscopy Reveals Li-Ion Solvation in Lithium Metal Batteries.
Small
January 2025
Guangxi Key Laboratory of Low Carbon Energy Materials, Guangxi Scientific and Technological Achievements Transformation Pilot Research Base of Electrochemical Energy Materials and Devices, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, P. R. China.
Inhomogeneous lithium (Li) deposition and unstable solid electrolyte interphase are the main causes of short cycle life and safety issues in Li metal batteries (LMBs). Developing a 3D structured matrix current collector and novel electrolyte are feasible strategies to tackle these issues. Ether-based electrolytes are widely used in LMBs.
View Article and Find Full Text PDFMicroscopic Characterization Based Synthesis of Silver Nanoparticles Using Merremia quinquefolia (L.) Hallier f. Leaf Extract: Their Biological Approaches and Degradation of Methylene Blue Dye.
Microsc Res Tech
January 2025
Department of Botany, Periyar University, Salem, Tamilnadu, India.
The green methods for the synthesis of silver nanoparticles (AgNPs) has developed popularity recently due to the low preparation costs, environmental friendliness, and non-toxicity of the precursors. In this study, AgNPs were synthesized using leaf extract from Merremia quinquefolia. Spectroscopic techniques were used for analyzing the functional groups, morphology, crystalline phase, and elemental composition of nanomaterials.
View Article and Find Full Text PDFHigh-performance boron nitride/graphene oxide composites modified with sodium thiosulfate for energy storage applications.
Nanoscale Adv
January 2025
School of Chemical Engineering, Yeungnam University 280 Daehak-Ro Gyeongsan 38541 Republic of Korea
Two-dimensional (2D) hybrid materials, particularly those based on boron nitride (BN) and graphene oxide (GO), have attracted significant attention for energy applications owing to their distinct structural and electronic properties. BN/GO composites uniquely combine the mechanical strength, thermal stability and electrical insulation of BN with the high conductivity and flexibility of GO, creating advanced materials ideal for the fabrication of batteries, supercapacitors and fuel cells. These hybrids offer synergistic effects, enhanced charge transport, increased surface area, and improved chemical stability, making them promising candidates for high-performance energy systems.
View Article and Find Full Text PDFMapping photoisomerization dynamics on a three-state model potential energy surface in bacteriorhodopsin using femtosecond stimulated Raman spectroscopy.
Chem Sci
January 2025
School of Physical Science and Technology, ShanghaiTech University Shanghai 201210 China
The process of proton translocation in , triggered by light, is powered by the photoisomerization of all--retinal in bacteriorhodopsin (bR). The primary events in bR involving rapid structural changes upon light absorption occur within subpicoseconds to picoseconds. While the three-state model has received extensive support in describing the primary events between the H and K states, precise characterization of each excited state in the three-state model during photoisomerization remains elusive.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!