Differential expression of gastrointestinal glutathione peroxidase (GI-GPx) gene during mouse organogenesis.

Anat Histol Embryol

College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.

Published: June 2011

AI Article Synopsis

  • GI-GPx, an antioxidant enzyme primarily found in the gastrointestinal tract of rodents, shows varying expression during mouse organ development, with higher levels in extraembryonic tissues like the placenta compared to the embryos.
  • In particular stages of embryonic development, GI-GPx mRNA is significantly present in areas such as the ectoplacental cone, neural tube, and heart, indicating its role in early organ formation.
  • The research suggests that GI-GPx may work alongside cGPx to protect embryos and their supporting tissues from oxidative stress during critical growth periods.

Article Abstract

Gastrointestinal glutathione peroxidase (GI-GPx) is an antioxidant enzyme that has been known to be restricted to the gastrointestinal tract in rodents. In an effort to determine the expression pattern of GI-GPx mRNA during organogenesis, quantitative real-time PCR and in situ hybridization for GI-GPx mRNA were conducted in whole embryos or each developing organ of mice. GI-GPx mRNA was expressed more abundantly in the extraembryonic tissues, including placenta than in embryos on embryonic days (EDs) 7.5-18.5 (P < 0.05). When compared with the expression levels of cytosolic GPx (cGPx) mRNA, GI-GPx mRNA levels were low in the embryos, but relatively high in the extraembryonic tissues (P < 0.05). According to the results of whole mount in situ hybridizations, GI-GPx mRNA was principally expressed in the ectoplacental cone, neural tube and fold, and primitive heart at EDs 7.5-8.5. At EDs 9.5-12.5, GI-GPx mRNA was abundantly expressed in nervous tissues such as the telencephalon, mesencephalon and dorsal neural tube and was also detected in the forelimb and hindlimb at EDs 10.5-12.5. In the sectioned embryos after ED 13.5, GI-GPx mRNA levels were high in the cerebral cortex, metanephric corpuscle, pancreatic ducts, surface epithelia of the skin, inner ear, and nasal conchae, gastrointestinal tract, liver, urinary bladder, airway passages of lung, and whisker follicles. These findings indicate that GI-GPx is not only spatiotemporally expressed in a variety of embryonic organs during organogenesis but also may perform a mutual compensatory role with the cGPx in the protection of embryos and extraembryonic tissues against the reactive oxygen species generated in ontogenetic periods.

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Source
http://dx.doi.org/10.1111/j.1439-0264.2010.01061.xDOI Listing

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