AI Article Synopsis
- The study aimed to clone a specific part of the leptospiral immunoglobulin-like protein (lig) gene and test its effectiveness as an antigen in an ELISA test for detecting antibodies to Leptospira in dogs.
- Using sera from 91 MAT-negative and 103 MAT-positive dogs, the ELISA demonstrated a sensitivity of 84.5% and a specificity of 76.9% compared to the standard MAT test.
- The findings suggest that the recombinant protein-based ELISA is a practical and efficient method for screening and confirming cases of canine leptospirosis.
Article Abstract
Abstract. The objectives of this study were to clone the conserved region of leptospiral immunoglobulin-like protein (lig) gene and evaluate the utility of the recombinant Lig as an ELISA antigen for detection of leptospiral antibodies in canine sera. Leptospira kirschneri serovar Grippotyposa strain Moskva V was chosen to be a target for cloning the conserved region of Lig gene. This assay was evaluated with canine sera (n = 91) that were MAT-negative (< 1:100 dilution) and sera (n = 103) that were MAT-positive (> or = 1:100 dilution) using 24 serovars. The ELISA showed a relative sensitivity as compared to MAT of 84.5% whereas the specificity was 76.9%. This assay is simple and can be routinely prepared in large amounts. It was concluded that the GST.Lig recombinant protein-based ELISA could be used as a screening test for serodiagnosis of canine leptospirosis with also for confirmation of MAT-positive test results.
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