Allozyme analysis of the temporal populations of Echinostoma revolutum collected from domestic ducks in Khon Kaen Province, Thailand.

Southeast Asian J Trop Med Public Health

Walai Rukhavej Botanical Research Institute, Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand.

Published: January 2011

AI Article Synopsis

  • Four distinct temporal populations of Echinostoma revolutum (ER1 to ER4) were collected from domestic ducks in Khon Kaen, Thailand during 2008, with samples taken in February, April, June, and October.
  • A study of 12 enzymes across 15 genetic loci revealed three polymorphic loci (G6pd-1, Me-1, and PepA-2), resulting in 28 unique genotypes, with Er6 being the most common among populations.
  • The genetic analysis showed 2-12% variation among populations, with certain genotypes like Er1, Er3, Er9, and Er12 displaying the most significant differences (12% variance), indicating intraspecific variation within E.

Article Abstract

Four temporal populations of Echinostoma revolutum (ER1, ER2, ER3, ER4) were collected from domestic ducks in Khon Kaen Province, Thailand during February-October 2008. The ER1, ER2, ER3 and ER4 were collected in February, April, June and October, respectively. The 12 enzymes encoding 15 loci were examined. Two loci were found in each of 3 enzymes, namely glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME) and peptidase valine-leucine (PEPA). Of these, three loci, namely, G6pd-1, Me-1 and PepA-2, were polymorphic. Genotypes were assigned for the specific allelic profiles detected at these three polymorphic loci. Twenty-eight genotypes were observed in the 4 temporal populations of E. revolutum. Three genotypes, Er22, Er23 and Er25, were found in all populations. The Er6 genotype occurred had the highest frequency of all the populations. These 28 genotypes were clustered into 3 groups with genetic differences of 2-12% among the loci. A cluster of genotypes (Er1, Er3, Er9 and Er12) showed the greatest genetic difference among the genotypes (12% difference). These results show intraspecific variation exists in E. revolutum populations in domestic ducks from Khon Kaen Province, Thailand.

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