The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 µg mL(-1), pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL(-1)), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and <1%, respectively.
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| http://dx.doi.org/10.2116/analsci.27.213 | DOI Listing |
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