The effect of column and eluent fluorination on the retention and separation of non-fluorinated amino acids and proteins in HPLC is investigated. A side-by-side comparison of fluorocarbon column and eluents (F-column and F-eluents) with their hydrocarbon counterparts (H-column and H-eluents) in the separation of a group of 33 analytes, including 30 amino acids and 3 proteins, is conducted. The H-column and the F-column contain the n-C(8)H(17) group and n-C(8)F(17) group, respectively, in their stationary phases. The H-eluents include ethanol (EtOH) and isopropanol (ISP) while the F-eluents include trifluoroethanol (TFE) and hexafluorosopropanol (HFIP). The 2 columns and 4 eluents generated 8 (column, eluent) pairs that produce 264 retention time data points for the 33 analytes. A statistical analysis of the retention time data reveals that although the H-column is better than the F-column in analyte separation and H-eluents are better than F-eluents in analyte retention, the more critical factor is the proper pairing of column with eluent. Among the conditions explored in this project, optimal retention and separation is achieved when the fluorocarbon column is paired with ethanol, even though TFE is the most polar one among the 4 eluents. This result shows fluorocarbon columns have much potential in chromatographic analysis and separation of non-fluorinated amino acids and proteins.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035200 | PMC |
| http://dx.doi.org/10.1016/j.jfluchem.2010.12.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Purification of mineral oil aromatic hydrocarbons and separation based on the number of aromatic rings using a liquid chromatography silica column. An alternative to epoxidation.
J Chromatogr A
January 2025
Analytical Chemistry Laboratory, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2, Gembloux 5030, Belgium. Electronic address:
The analysis of mineral oil aromatic hydrocarbons (MOAH) in vegetable oils is currently associated with high uncertainty due to various factors ranging from sample preparation to data interpretation. One significant factor is the coelution of biogenic compounds of terpenic origin with the MOAH fraction during chromatographic analysis. The common purification method is epoxidation, a chemical reaction that changes the polarity of the interferences, allowing their separation from MOAH.
View Article and Find Full Text PDFEnhanced nano-LC-MS for analyzing dansylated oral cancer tissue metabolome dissolved in solvents with high elution strength.
Anal Chim Acta
February 2025
Department of Biochemistry and Molecular Biology, Chang Gung University, Taoyuan, 333, Taiwan; Clinical Proteomics Core Laboratory, LinKou Chang Gung Memorial Hospital, Taoyuan, 333423, Taiwan. Electronic address:
Background: Tissue metabolomics analysis, alongside genomics and proteomics, offers crucial insights into the regulatory mechanisms of tumorigenesis. To enhance metabolite detection sensitivity, chemical isotope labeling (CIL) techniques, such as dansylation, have been developed to improve metabolite separation and ionization in mass spectrometry (MS). However, the dissolution of hydrophobic derivatized metabolites in solvents with high acetonitrile content limits the use of liquid chromatography (LC) systems with small-volume reversed-phase (RP) columns.
View Article and Find Full Text PDFDetermination of the appropriate extraction method for bound aroma compounds from strawberry and analysis of aroma substances in strawberry fruits of different varieties and developmental stages.
Food Chem
January 2025
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China. Electronic address:
Strawberries are rich in unique aroma substances, which include bound and free aroma compounds. Unlike the free aroma, the appropriate extraction and analytical methods for bound aroma compounds in strawberries remain unclear. In the present study, we compared three extraction methods for bound aroma compounds of strawberries and performed the single factor experiment for optimizing the hydrolysis method, process parameters, and response surface analysis.
View Article and Find Full Text PDF4D-printed programmable sample-/eluent-actuated solid-phase extraction device for trace metal analysis.
Anal Chim Acta
January 2025
Department of Chemistry, National Chung Hsing University, Taichung City, 402202, Taiwan, ROC. Electronic address:
Background: To integrate valves, manifolds, and solid-phase extraction (SPE) columns into a compact device is technically difficult. Four-dimensional printing (4DP) technologies, employing stimuli-responsive materials in three-dimensional printing (3DP), are revolutionizing the fabrication, functionality, and applicability of stimuli-responsive analytical devices that can show time-dependent shape programming to enable more complex geometric designs and functions. However, 4D-printed stimuli-responsive actuators and valves utilized to control flowing streams in SPE applications remain rare.
View Article and Find Full Text PDFValidated HPLC-UV Method for the Quantification of a Novel BCr-Abl 1 Inhibitor, Vodobatinib, in Rat Plasma: Application to a Pharmacokinetic Study.
Biomed Chromatogr
February 2025
Drug Metabolism and Pharmacokinetics, Laxai Life Sciences Pvt. Ltd., Shamirpet, Hyderabad, India.
Vodobatinib is a Bcr-Abl 1 inhibitor, currently entering into Phase 2 clinical trials as a potential drug to treat glioblastoma patients. In the present work, a validated high-performance liquid chromatography (HPLC) detection method for the quantification of vodobatinib in rat plasma was established. Sample preparation involved liquid-liquid extraction method.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!